基于高压液相色谱法的地沟油聚类分析方法研究

The clustering analysis of the illegal cooked oil based on high pressure liquid chromatography

目的建立基于高压液相色谱法的地沟油聚类分析方法,对地沟油进行行之有效的测定。方法假设目标油样的主要成分分为甘油三酯和其他杂质两大类,通过对其MALDI-TOF质谱峰的分析及高压液相色谱峰(HPLC)的聚类判别,实现地沟油的分析检测。首先利用MALDI-TOF质谱对油样进行初步分析;然后根据出峰时间选取了14个特征高压液相色谱峰,得到其面积百分比,并对其进行标准化处理;采用聚类分析技术将41个已知油样聚成六大类,建立样品数据库;对未知样品数据进行判别分析,可以对其进行检测。结果该方法可以检测到食用油中掺入10%的地沟油含量。将该方法用于26个盲样,结果显示,准确率为92.3%。结论该方法可以对地沟油进行有效地测定。

@@@@Objective This paper provides an effective testing method of the illegal cooked oil. Methods The triglyceride and other impurities in oil were as targets to be analyzed using MALDI-TOF mass spectrum and high pressure liquid chromatography (HPLC). First, MALDI-TOF MS was employed for preliminary analysis of oil samples; then 14 feature HPLC peaks according to the peak time and area percentage were used to carry on the standardized processing. By using the cluster analysis technology, the 41 known oil samples were gathered into 6 categories and the sample databases were established, and the discriminate analysis of the unknown samples enabled us to inspect these oils. Results Qualified edible oil mixed with 10% of the illegal cooked oil could be detected. Using this method for a blind test of 26 samples, the results showed that the total accuracy rate was 92.3%. Conclusion This method is an effective reference method for illegal cooked oil detection.