荧光定量PCR方法检测畜肉食品中鸭源性成分

Rapid detection of duck-derived materials in meat products by real time PCR

目的建立一种快速、特异、灵敏的鸭源性成分检测方法。方法本研究以鸭线粒体基因全序列为靶位点设计引物和探针,进行荧光定量PCR扩增,建立鸭源性成分检测方法;以常见畜禽肉包括羊肉、牛肉、鸡肉、鹅肉、猪肉、兔肉、马肉、鹿肉等参考动物物种作特异性检测;以50 mg/kg羊肉DNA作为稀释液对鸭肉DNA进行梯度稀释,做灵敏度检测。结果该方法能够有效对鸭源性成分进行快速检测,具有较强的特异性,灵敏度较高(可达0.1μg/kg)并且羊肉成分的存在对鸭肉灵敏度检测没有影响。结论该方法特异性强,灵敏度高,可以快速、准确检测畜肉食品中含有的鸭源性成分。

Objective To establish a rapid, specific, sensitive method for detection of duck derived materials. Methods The real-time PCR method for detection of duck-derived materials in meat products was established using forward and reverse primers and probe corresponding to the mitochondrial gene. The specific detection was carried out by livestock and poultry meat including mutton, beef, chicken, goose, por-cine, rabbit meat, horse meat, venison and other reference animal species. Sensitivity detection was done using the 50 mg/kg mutton DNA as diluent for gradient dilution of duck DNA. Results This method can effectively detect duck derived materials, with a strong specificity and high sensitivity (up to 0.1μg/kg), and without obvious influence by mutton components on duck sensitivity detection. Conclusion This method has a strong specificity and high sensitivity, and can rapidly and accurately detect the duck-derived compo-nents in meat products.