高盐辣椒坯中一株耐盐乳杆菌的分离及鉴定

Isolation and identification of salt-tolerance lactic acid bacterium from high-salt chili

目的采用选择性的MRS培养基,从湖南地区特色的盐坯辣椒中分离筛选出耐盐优势菌。方法得到一株能在18%NaCl培养基中生长的耐盐菌进行了生理生化实验,通过菌落PCR扩增其16S rDNA基因序列并测序,经过序列比对构建系统发育树。结果这株菌有较好的产乳酸、耐盐的能力,经过形态观察和生理生化试验验证,属于乳酸杆菌。结论经过16S rDNA进一步鉴定为植物乳杆菌。

Objective To isolate some salt-tolerance strains with different colony morphologies from Hunan characteristic fermented chilli samples using selected MRS medium. Methods One isolated strain which could grow on an 18%NaCl salt tolerant bacteria medium was identified base on the physiological and biochemical experiments with 16S rDNA sequencing and phylogenetic tree analyses. Results This strain had a good ability of producing lactic acid and high tolerance to salt. Conclusion This strain was identified as Lactobacillus plantarum through 16S rDNA sequence analysis.