45例儿童急性淋巴细胞性白血病流式细胞术免疫分型的研究

Investigation of immunophenotype in 45 cases of children with acute lymphoblastic leukemia via flow cytometry

目的研究儿童急性淋巴细胞性白血病(ALL)免疫表型的特点。方法采用流式细胞仪对45例初治儿童急性淋巴细胞性白血病进行免疫表型分析。结果①45例ALL中T淋巴细胞性ALL(T-ALL)5例(11.1%),B淋巴细胞性ALL(B-ALL)36例(79.9%);ALL1型(L1)9例(20%),ALL2型(L2)27例(60%),ALL3型(L3)5例(11.1%);②45例ALL中4例符合急性混合性白血病(HAL)的诊断标准(8.9%),25例(55.6%)为髓系抗原阳性的ALL(My+ALL),其中CD13阳性率最多,为44.4%(20例),CD33为22.2%(10例),CD15为22.2%(10例)。B-ALL中My阳性率为55.6%,T-ALL中My阳性率为20%,有明显差异;L1中My阳性率为22.2%(2/9),L2中My阳性率为70.4%(19/27),L3中My阳性率为40%(2/5),L2中My阳性率明显高于L1和L3(P<0.05)。结论结合免疫表型分析与细胞形态学检验能为儿童急性淋巴细胞性白血病的诊断提供更可靠和更有价值的分型依据。

Objective To study the character of immunophenotype in children with acute lymphoblastic leukemia(ALL).Methods To assay the immunophenotype in 45 children with acute leukemia by flow cytometer.Results ① In 45 cases ALL T-ALL was 11.1%,B-ALL was 79.9%;ALL1 20%,ALL2 60.0%,ALL3 11.1%;② In 45 cases ALL,four children was diagnosed HAL by immunophenotype and the positive rate of myeloid antigen was 55.6%.While the positive rate of CD13 was 44.4%,CD33 was 22.2%,CD15 was 22.2%,respectively.The positive rate of myeloid antigen in B-ALL(55.6%) was significantly higher compare with T-ALL(20%)(P<0.05);the positive rate of myeloid antigen in L2(70.4%) was significant higher than in L1(22.2%) and L3(40%)(P<0.05).Conclusion Immunophenotyping,combined with morphology can provide more reliable and valuable evidence in the diagnosis of children with lymphoblastic leukemia.