摘要
To screen specific antitumor drugs targeting telomerase catalytic subunit (hEST2), 12 different hEST2 antisense oligonucleotides were designed based on hEST2 mRNA second structure and transfected into tumor cell lines by the lipofectin-mediated method. Cell growth activity was evaluated by MTT assay. HEST212 was picked out and its specificity, antitumor tree and continuous effect were analyzed. The results showed that hEST212 had promising anti-tumor activity in vitro, hEST2 can be used as a pratical target and an antisense drug candidate for cancer.
To screen specific antitumor drugs targeting telomerase catalytic subunit (hEST2), 12 differenthEST2 antisense oligonucleotides were designed based on hEST2 mRNA second structure and transfected into tumor cell lines by the lipofectin-mediated method. Cell growth activity was evaluated by MTT assay.hEST212 was picked out and its specificity, antitumor tree and continuous effect were analyzed. The results showed thathEST212 had promising antitumor activityin vitro, hEST2 can be used as a pratical target and an antisense drug candidate for cancer.
基金
This work was supported in part by the National Natural Science Foundation of China (Grant No. 39870879)
the State "863" High-Tech Project (Grant Nos.102-08-04-01 and 2001AA215261)
the Key Project from the Committee of Beijing Science Technology (Gra
