动物细胞培养用生物反应器及其力学环境

Bioreactors for Animal Cell Culture and Their Mechanical Environment

动物细胞体外培养时 ,生物反应器是整个培养过程的关键设备 ,为细胞提供一个适宜的生长环境 ,使之快速增殖并形成所需的生物组织制品。由于动物细胞在其形态结构、培养方法以及所需的力学环境等方面均不同于微生物细胞 ,因而传统的微生物反应器显然已不适用于动物细胞大规模培养 ,特别是组织工程的需要 ,促使新型生物反应器的研究与开发。本文针对动物细胞培养的基本特点 ,综述了动物细胞培养用生物反应器 。

Bioreactor is a key device during in vitro animal cell culture. It provides an appropriate growth environment for the cells, enabling them to proliferate and become the final bio-product. Since animal cells are different from microbial cells in morphology, culture skill needed, and mechanical stimulation needed, the traditional microbial bioreactors are not suitable for the large-scale animal cell culture any more, and many new bioreactors are developed. This paper reviews the bioreactors particularly designed for the animal cell culture, and addresses the mechanical loads on the cultured cells.