国人睾丸、附睾和输精管的水解酶的组织化学观察

ENZYME HISTOCHEMICAL OBSERVATION OF HYDROLASES IN HUMAN TESTIS EPIDIDYMIS AND VAS DEFERENS

本文报导国人睾丸、附睾和输精管的ＮＳＥ，ＡＣｈＥ，ＣｈＥ，ＡＬＰ，ＡＣＰ，５＇－Ｎａｓｅ，Ｇ－６－Ｐａｓｅ，β－ＧＡ，β－ＧＲ，ＡＰ－Ｍ，ＡＴＰａｓｅ和ＴＰＰａｓｅ水解酶的组织化学活性、结果显示：睾丸曲细精管的ＡＬＰ，５＇－Ｎａｓｅ和ＡＴＰａｓｅ；睾丸间质细胞的ＮＳＥ，ＡＬＰ，ＡＴＰａｓｅ和ＡＣＰ；睾丸间质中的ＮＳＥ，ＡＬＰ和ＡＴ－Ｐａｓｃ；睾丸输出小管和附睾管上皮的ＮＳＥ，ＡＬＰ，ＡＣＰ，５’－Ｎａｓｅ，β－ＧＡ，β－ＧＲ，ＡＴＰａｓｅ和Ｔｐｐａｓｅ；附睾头部间质中的ＮＳＥ，ＡＣｈＥ，ＡＬＰ，和ＡＴＰａｓｅ；输精管上皮细胞的ＡＴＰａｓｅ的酶活性均呈强阳性或极强阳性。说明人类睾丸、附睾和输精管含有丰富的水解酶，尤其是附睾头部的输出小管、附睾管和头质均含有种类多活性高的水解酶，在精子的功能成熟上起了重要的作用，提示其可能作为生育与不育诊治中的重要指标。

The testes,eptididymides and vas deferens of 13 cases of sudden unexpected death donors,age from 19-38,were studied in this investigation with histochemical methods.12 kinds of hydrolases in the testes,epididymides and vas deferenswere half quantitatively observed with light microscopes.The results showed enzymes activities were strong positive orhighly strong positive in the following tissues and cells: ALP,5'-Nase and ATPase in seminiferous tubules;NSE,ALP,ATPase and ACP in Leydig's cells; NSE,ALPase and ATPase in interstitial tissue of the testis; NSE,ALP,ACP,5'-Nase,β-GA,β-GR ATPase and TPPase in the epithelia of ductuli efferentes and ductus epididymidis; NSE,AChE,ALP and ATPase in the interstitial tissue of the head of epididymis;ATPase in the epithelia of vas deferens.Our study indicates that many hydrolases exist in the caput epididymidis,which may play an important role in the maturation of human sperm. So it is also suggested that those hydrolases activities may be employed as indexes in the diagnosis and treatment sterilization as well as in the investigation of new contraceptive methods.