中药化学成分的液质联用鉴定法探讨

Identification of chemical composition in traditional Chinese medicine by liquid chromatography-mass spectrometry

目的研究采用液质联用法鉴定中药化学成分。方法用2种方法对86种中药的化学成分进行鉴定。观察组采用液质联用方法,对照组采用色谱法。对比2组鉴定方法的检测效果、准确率以及鉴定时间等。结果观察组优者占比76.74%(66/86),优良率为90.70%(78/86),均显著高于对照组(P<0.05)。观察组成功鉴定83例,成功率为96.51%,显著高于对照组(P<0.05)。观察组鉴定时长、校正后保留时间误差均显著少于对照组(P<0.05)。结论液质联用法鉴定中药化学成分准确率高,鉴定时间短,适应范围广。

Objective To identify the chemical components of traditional Chinese medicine by liquid chromatography-mass spectrometry.Methods Chemical components of 86 traditional Chinese medicines were identified by two methods,observation group with LC-MS method and control group with traditional LC methods.The identification efficacy,accuracy,and identification time between two methods were compared. Results The excellent rate in observation group was 76.74% (66/86),the excellent and good rate was 90.70%(78/86),which were significantly higher than those in control group (P<0.05 ).83 kinds of traditional Chinese medicine were successfully identified in observation group,the success rate was 96.5 1%,significantly higher than control group(P<0.05).The identification tine,retention time after correction of errors in observation group were significantly less than control group(P<0.05 ).Conclusion LC-MS has high accuracy,short identification time and wide adaptation range in identification of chemical components of traditional Chinese medicine.