“猪康散”保健用药对人工感染猪繁殖与呼吸综合征病毒仔猪血清细胞因子含量的影响

Healthcare effects of Zhukangsan on the levels of cytokines of piglets artificially challenged by PRRSV

选取28日龄健康杜长大三元杂交仔猪40头,随机分为4组,每组10头。36~39日龄中药组(Ⅲ、Ⅳ组)分别在基础日粮中添加0.5%、1.0%'猪康散',40日龄除阴性对照组(Ⅰ组)外均滴鼻接种PRRSV SCM株1.0mL。于40、45、50、55、60日龄每组选取4头仔猪,前腔静脉采血测定血清细胞因子(IL-2、IL-6、IFN-γ、TNF-α)的变化。结果显示,在整个试验期间,除0.5%中药组40日龄仔猪血清中IL-2含量与阴性对照组差异不显著外(P>0.05),0.5%和1.0%中药组仔猪血清中IL-2、IL-6、IFN-γ、TNF-α的含量均显著高于阴性对照组和阳性对照组(P<0.01或0.05);除两中药组40日龄仔猪血清中IL-6含量差异不显著外(P>0.05),1.0%中药组仔猪血清中IL-2、IL-6、IFN-γ、TNF-α含量均显著高于0.5%中药组(P<0.01或0.05)。结果表明,在断奶仔猪基础日粮中添加0.5%和1.0%质量分数的'猪康散'能明显提高人工感染PRRSV仔猪血清中IL-2、IL-6、IFN-γ、TNF-α的含量,从而提高仔猪机体免疫功能,达到抗PRRSV的能力。

40piglets of 28-day-old were divided randomly into 5groups treated with virus and negative group(Ⅰ),positive group(Ⅱ),0.5%(Ⅲ),and 1.0%(Ⅳ).Group Ⅲ and Ⅳ were given drug(Zhukangsan)at 0.5% and 1.0% in the forage at 36 to 39-day-old,respectively,then all groups except group Ⅰ were PRRSV SCM at 40-day-old.Four piglets from each group were selected randomly for obtaining blood samples,there were no differences(P>0.05)between the level of IL-2in 0.5% 'Zhukangsan'group and that of negative group on day 40,the levels of IL-2,IL-6,IFN-γ,TNF-αof 0.5% and 1.0% 'Zhukangsan'groups had significantly differences(P<0.01 or 0.05)compaked to those of negative group and positive group.there were on differences(P>0.05)between 0.5% 'Zhukangsan'group and 1.0% 'Zhukangsan'group on the level of IL-6on day 40,the levels of IL-2,IL-6,IFN-γ,TNF-αof 1.0% 'Zhukangsan'group were markedly higher(P< 0.01 or 0.05)than those of 0.5% 'Zhukangsan'group.The results indicated that0.5% and 1.0% complex chinese crude drug 'Zhukangsan'in the forage could enhance the immune responses by improving the levels of IL-2,IL-6,IFN-γ,TNF-αof piglets artificially challenged by PRRSV,thus build up resistance of piglets to PRRSV.