牡蛎粗多糖对脂多糖刺激仔猪NF-κB信号通路相关基因转录的影响

Explore Mechanism of Oyster Crude Polysaccharide Alleviated Immune Stress on Weanling Piglets

旨在初步探讨牡蛎粗多糖对免疫应激仔猪NF-κB信号通路相关基因的影响。选取30头28日龄左右的杜长大三元阉公仔猪,分为5个处理组,即空白对照组(Ⅰ)、免疫应激对照组(Ⅱ)以及牡蛎粗多糖低(Ⅲ)、中(Ⅳ)、高(Ⅴ)剂量组,每组6头猪。Ⅰ、Ⅱ组饲喂基础日粮,Ⅲ、Ⅳ、Ⅴ组分别饲喂含0.5%、0.8%、1.2%牡蛎粗多糖的基础日粮。试验饲养30d后,Ⅱ、Ⅲ、Ⅳ、Ⅴ组腹腔注射100μg·kg-1 LPS,Ⅰ组注射等量的生理盐水。各组于注射后3h剖检取肝、脾、肾上腺、淋巴结和胸腺等组织,检测TLR-4、p300和p50的相对转录水平。结果:(1)与Ⅰ组相比,Ⅱ组p300相对转录水平在淋巴结中极显著性降低,其他指标在各器官中极显著升高(P<0.01)。Ⅲ组TLR-4在淋巴结中,p50在肾上腺中和p300在肝、肾上腺及淋巴结中的相对转录水平均极显著降低(P<0.01);p50在肝、胸腺和脾中,p300在胸腺和脾中相对转录水平极显著升高(P<0.01)。Ⅳ组TLR-4在淋巴结和脾中,p300在肝、肾上腺和淋巴结中相对转录水平显著降低(P<0.05或P<0.01);TLR-4在肝、肾上腺和胸腺中,p50在肝和胸腺中相对转录水平均显著升高(P<0.05或P<0.01)。Ⅴ组TLR-4在脾,p300在肝、淋巴结、胸腺和肾上腺中相对转录水平显著降低(P<0.05或P<0.01);TLR4在肝、肾上腺和淋巴结中,p50在肝、胸腺和肾上腺相对转录水平显著升高(P<0.05或P<0.01)。(2)与Ⅱ组相比,除Ⅴ组TLR-4在淋巴结中的差异不显著外,Ⅲ、Ⅳ、Ⅴ组TLR-4的相对转录水平在各器官中均显著降低(P<0.05或P<0.01);p50的相对转录水平在肾上腺、淋巴结和脾中极显著降低(P<0.01),Ⅲ组p50的相对转录水平在肝中显著降低(P<0.01),在胸腺中极显著升高(P<0.01),Ⅴ组p50的相对转录水平在胸腺中差异极显著降低(P<0.01);p300的相对转录水平在肝、肾上腺、胸腺和脾中极显著降低(P<0.01);在淋巴结中,Ⅳ组p300的相对转录水平极显著升高(P<0.01)。牡蛎粗多糖可以缓解免疫应激仔猪器官中NF-κB信号通路相关基因的转录水平。

This study was designed to explore the impact of Oyster Polysaccharides on immune stress piglets of NF-κB signaling pathway-related genes A total of thirty Duroc × Landrace ×Yorkshire castrated piglets of 28±1dwere randomly allocated into five groups,namely Blank control(Ⅰ),Immunological stress control(Ⅱ)and Oyster polysaccharide Low(Ⅲ),Medium(Ⅳ)and High(Ⅴ)dose group,with six replicates per group according to the principle of similar weight.Piglets were fed basal diet(Control)or 0.5%,0.8%,1.2% OPS(OPS Low,Medium andHigh dose group)for 30 days.The piglets were injected i.p with a dose of Escherichia coli LPS(100μg·kg-1 BW)except the Blank control which were injected with the same dose of normal saline.Three hours later,the liver,spleen,adrenal gland,lymph nodes and thymus were collected for detecting the relative transcription level of TLR-4,p50 and p300.Results were as follows:(1)Compared with the GroupⅠ,p300 relative transcript levels of GroupⅡ were significantly decreased in Lymph Node,while the other indicators were significantly increased in various organs(P<0.01).The relative transcript levels of TLR-4in lymph nodes,p50 in adrenal gland and p300 in liver,adrenal gland and lymph nodes were significantly decreased(P<0.01)while the relative transcript levels of p50 in adrenal gland and p300 in liver,adrenal gland and lymph node were significantly increased(P<0.01)in GroupⅢ than GroupⅠ.The relative transcript levels of TLR-4in lymph nodes and spleen,p300 in liver,adrenal gland and lymph nodes were significantly decreased(P<0.05 or P<0.01)while the relative transcript levels of TLR-4in liver,adrenal gland and thymus and p50 in liver,thymus were significantly increased(P<0.05 or P<0.01)in GroupⅣ than GroupⅠ.The relative transcript levels of TLR-4in spleen,p300 in liver,lymph nodes,thymus and adrenal gland were significantly decreased(P<0.05 or P<0.01)while the relative transcript levels of TLR-4in liver,adrenal gland and lymph nodes and p50 in liver,thymus and adrenal gland were significantly increased in Group Ⅴthan Group Ⅰ(P<0.05 or P<0.01).(2)Compared with the Group Ⅱ,the relative transcription of TLR-4levels were significantly decreased in these organs of GroupⅢ,ⅣandⅤ(P<0.05 or P<0.01)other than in lymph node of Group Ⅴ(P>0.05).The relative transcription of p50 levels were significantly decreased in adrenal glands,lymph glands and spleen of GroupⅢ,Ⅳ,andⅤ(P<0.01);and were significantly decreased in liver and significantly increased in thymus of GroupⅢ(P<0.01);and that were significantly increased in thymus of Group Ⅴ(P<0.01).The relative transcription of p300 mRNA levels were significantly decreased in liver,adrenal glands,thymus and spleen of GroupⅢ,Ⅳ,andⅤ(P<0.01);and that were significantly increased in lymph nodes of Group Ⅳ(P<0.01).Oyster polysaccharides can relieve stress piglets transcription of NF-κB signaling pathway-related genes.