蒺藜苜蓿LEA基因家族全基因组分析

Genome-wide analysis of LEA gene family in Medicago truncatula

胚胎发育晚期丰富蛋白(LEA)是一类在种子胚胎发育后期特异表达并受发育阶段及脱水信号调节的脱水保护蛋白,在响应植物干旱、低温、高盐等逆境胁迫中具有重要功能。本研究利用生物信息学手段,在全基因组水平对蒺藜苜蓿(Medicago truncatula)LEA基因家族进行分析,并对其进化、基因结构、进化压力、染色体定位及基因表达模式进行了系统分析。本研究共筛选出23个蒺藜苜蓿LEA家族基因,可分为8个亚家族。基因定位结果表明,23个蒺藜苜蓿LEA基因分布于除6号染色体外的其他7条染色体上,但分布不均匀;家族成员外显子数目都不超过两个,结构简单。不同组织表达谱分析结果显示,LEA家族基因具有不同的组织表达模式,并受干旱逆境胁迫调控。本研究结果可为蒺藜苜蓿LEA基因家族的功能分析奠定基础。

Late embryogenesis abundant( LEA) family proteins are expressed abundantly at the late stage of embryonic development of plant seeds,and play important roles in responding to various environmental stress,such as drought,low temperature and high salinity.At present,the phylogenetic studies of LEA gene family in Medicago truncatula have not been reported yet.In this study,with the application of bioinformatics methods,the LEA family genes of M.truncatula were identified through the whole genome,and the system evolution,gene structure,evolutionary pressure,chromosomal location and gene expression patterns were further analyzed.A total of 23 LEA genes were systematically identified from M.truncatula and classified into 8 subfamilies.Gene location results showed that these 23 LEA genes were distributed unevenly on 7 chromosomes; The exon numbers of all the genes were no more than two,indicating a simple gene structure of this gene family.The expression profiles of M.truncatula LEA genes showed a characteristic of temporal and tissue specific,and regulated by drought stress.This study provides a theoretical foundation for verifying the function of LEA genes in M.truncatula.