猪伪狂犬病病毒HLJ8株的分离与鉴定

Isolation and identification of swine pseudorabies virus HLJ8 strain

2013年黑龙江省某猪场发生仔猪死亡并且伴有神经症状,采集死亡猪脑组织,经PCR检测、病毒电镜观察,确诊为伪狂犬病病毒(PRV)感染,遂将该毒株命名为HLJ8。进一步对病毒gE基因的序列分析表明,该毒株与2012年的中国分离株在同一个相对独立的分支中,氨基酸序列的同源性在94%~100%之间;与2011年之前分离的毒株的亲缘关系较远。小鼠毒力试验结果表明,高剂量(1×103 PFU/mL)的HLJ8株可以引起小鼠死亡以及皮肤瘙痒等典型的PRV感染症状,但其毒力略低于经典强毒株PRV SC株。

The disease was occurred on a pig farm in Heilongjiang in 2013,with large number of deaths of piglets accompanied by neurological symptoms.By detecting with PCR and viral isolation,apseudorabies virus(PRV)strain named HLJ8 was isolated.Further analysis showed that PRV gE sequence of HLJ8 strain was in a relatively independent clade with Chinese isolates from 2012,and with a homology from94%to 100% at amino acid level.BALB/c mice infected with high doses(1×103 PFU/mL)HLJ8strain were died,accompanied by typical PRV infection symptoms such as itchy skin.However,the pathogenicity of HLJ8 strain was slightly lower than PRV SC,a classical virulent strain.