BEL-7402肝癌细胞株的生长抑素受体放射分析

Analysis of somatostatin receptors of BEL-7402 cell line

目的 探讨生长抑素受体在肝癌细胞株中的表达。方法 用12 5I [Tyr3 ] octreotide作为生长抑素受体的放射性配体 ,检测肝癌细胞株BEL 74 0 2上受体位点数和亲和常数。结果 受体放射分析显示肝癌细胞株BEL 74 0 2上的生长抑素受体与12 5I [Tyr3 ] octreotide的结合可以达到较满意的饱和曲线 ;表达的生长抑素受体位点数为 1 0× 10 5个 /细胞 ,位点数多 ;与octreotide的平衡解离常数 (Kd)为 0 2 4× 10 -9mol/L ,亲和力强。结论 肝癌细胞株上有生长抑素受体表达 ,密度高 ,且与临床常用药octreotide有较好的亲和力。

Objective To evaluate the expression of somatostatin receptors in HCC cell line. Methods Taking 125 I--octreotide as the radioligands, HCC BEL-7402 cell line was investigooted for the receptor binding points and affinity constant. Results Receptor analysis study showed satisfactory combination between the somatostatin receptors of HCC BEL-7402 cell line and 125 I--octreotide. The expressed somatostatin receptors on HCC cell line were numerous(B max =1.0×105/cell) and had strong affinity to octreotide (K d=0.24×10 -9 mol/L). Conclusion Somatostatin receptors were expressed on HCC cell line. The receptor's density was high and the affinity to octreotide was strong.