氧化型低密度脂蛋白与2型糖尿病大鼠主动脉病变的关系

The Study of the Significance of Oxidized Low Density Lipoprotein in the Process of Aorta Disease in the Rats with Type 2 Diabetes Mellitus

为探讨氧化型低密度脂蛋白在 2型糖尿病大鼠主动脉病变中的作用。将大鼠随机分成正常对照、高糖高脂和糖尿病三组。分别于胰岛素抵抗期、糖尿病形成后第 10周末和第 2 0周末处死大鼠 ,应用微机图像分析系统对主动脉内膜—中膜厚度进行测量 ;应用酶联免疫吸附法测定血清氧化型低密度脂蛋白 ;应用DCA2 0 0 0测定糖化血红蛋白。结果发现 ,糖尿病形成后第 10周末糖尿病组氧化型低密度脂蛋白 (6 .5 7± 0 .4 9)显著高于正常对照组(4 .2 0± 0 .4 3)和高糖高脂组 (4 .92± 1.2 0 ) (P <0 .0 5 ) ,后两组无明显差异 ,且糖尿病组内膜—中膜厚度 (370 .8± 34.2 )显著高于正常对照组 (2 84 .8± 33.8)和高糖高脂组 (337.2± 37.6 ) ,高糖高脂组显著高于对照组 (P <0 .0 5 )。这种状态一直持续到实验结束。相关分析发现内膜—中膜厚度与氧化型低密度脂蛋白呈显著正相关 (r=0 .85 ,P <0 .0 5 ) ,糖化血红蛋白与氧化型低密度脂蛋白呈显著正相关 (r=0 .87,P <0 .0 5 ) ,内膜—中膜厚度与糖化血红蛋白相关不明显 (P >0 .0 5 )。此结果提示 ,氧化型低密度脂蛋白在 2型糖尿病大鼠主动脉病变中起重要作用 ,能促进大血管粥样硬化的发生发展。

Aim To study the role on oxidized low density lipoprotein (ox-LDL) in the process of aorta disease on type 2 diabetes mellitus rats. Methods Wister rats were divided into three groups radomly (normal controls,fed on high fat and high glucose diets,and diabetes group). In the insulin resistance (IR) stage, 10 th week and 20 th week after developed diabetes, the intima-media thickness (IMT) were detected using image analysing system, ox-LDL and GhbA1C were examined by ELAsA. Results In the 10th week 10th week and IMT in group C were significantly higher than in group A and B (P<0.05), there was not obviously different between group A and B, whereas IMT in group B were marked higher than in group A, this condition continue to the end of the experiments, meanwile we found the level of IMT was significantly correlated with ox-LDL (r=0.85,P<0.05) And ox-LDL was significantly correlated with HbA1C (r=0.87, P<0.05) by multiple regression analyse,whereas IMT was not significantly correlated with HbA1C. Conclusions ox-LDL has a significant role in the aorta disease of type 2 diabetes mellitus rats,It helps the development of macrovascular damage.