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影像学技术在组织工程骨修复山羊大段骨缺损长时间观察中的应用 被引量:4

Application of radiology in the long-term follow-up of repairing large bone defects of goats with tissue engineering technique
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摘要 目的探讨放射性核素骨显像(ECT)、X线、CT等影像学技术在组织工程骨修复山羊大段负重骨骨缺损远近期实验观察中的应用价值。方法中国青山羊15只,制备单侧胫骨2cm的骨膜与骨缺损,缺损内植入组织工程骨犤CHAP珊瑚羟基磷灰石+经诱导分化的骨髓基质干细胞(bonemarrowstromacells,BMSCs)犦,术后ECT、X线、CT等影像学手段检查,评价骨缺损修复情况及各种手段的应用价值。其中在早期术后2、4、8周行ECT检查,4、8、12周行X线检查。远期在术后2、3、6个月行CT检查,术后6、12、18个月行X线。结果术后影像学手段检测表明,组织工程骨组可以修复2cm的骨缺损,ECT显示在术后2个月内骨再生和再血管化进展顺利,X线和CT显示术后组织工程骨成骨呈渐进性和偏心性,12个月以后组织工程骨与山羊胫骨牢固愈合,并开始塑形。结论组织工程骨具有良好的修复山羊大段骨缺损的能力,在这一过程中影像学技术提供了理想的直观依据。 Objective To study the values of ECT, X-ray, CT scanning in the long-term follow-up of repairing large bone defect of goats with tissue engineering technique. Methods Left tibia diaphyseal defects 2cm in length were made in 15 Chinese goats. The defects were filled with coral hydroxyapatite (CHAP) plus bone marrow stroma cells (BMSc). The repairing effects of the defects were evaluated by ECT, X-ray and CT scanning. At the early stage radionuclide bone imaging was used to monitor the revascularization of the implants at 2, 4, and 8 weeks after operation. X-ray examination was used at 4, 8, and 12 weeks after operation. In the long-term follow-up x-ray was applied at 6, 12, 18 months postoperatively and CT scanning at 2, 3, and 6 months postoperatively. Results Tissue engineered bone could repair large bone defects of goats. ECT showed good bone regeneration and revascularization within 2 months postoperatively. X-ray and CT scanning showed eccentric and gradual bone regeneration. 12 months later, tissue engineered bone healed firmly with caprine tibia and started to mould. Conclusions Tissue engineered bone can repair the large bone defects of goats. Radiology is a good method to monitor the process.
出处 《中华创伤骨科杂志》 CAS CSCD 2004年第7期752-755,共4页 Chinese Journal of Orthopaedic Trauma
基金 国家高技术研究发展计划(863计划)(2003AA205010)
关键词 骨组织工程 山羊 成骨 影像学 Tissue engineering Goat Osteogenesis Radiology
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