油茶离体培养诱导再生植株的研究

Study on Organogenesis and Induction of Regeneration Plant of Camellia oleifera

采用普通油茶Camelliaoleifera优良无性系湘林4号为实验材料,研究了离体条件下带芽的茎段、幼胚、子叶的愈伤组织的形成和植株再生技术。茎段离体培养以MS为基本培养基,附加6-BA3.0mg·L-1+NAA1.0mg·L-1对芽进行诱导,诱导率达到83.33%;继续在此培养基上继代得到丛生芽,增殖比例为1∶20。对于幼胚和子叶,附加2,4-D2.0mg·L-1+KT1.0mg·L-1诱导愈伤组织得到很好的效果,将愈伤组织转到附加6-BA3.0mg·L-1+NAA0.05mg·L-1和6-BA2.5mg·L-1+IAA1.5mg·L-1的培养基上诱导出不定芽,诱导率达到90%以上。芽苗增殖以MS+6-BA2.5mg·L-1+IAA1.5mg·L-1的培养基为好。将无菌苗在MS培养基(附加NAA7mg·L-1)上诱导生根,生根率达93%。

The excellent asexual Camellia oleifera named as Xianglin 4th served as experiment material. The formation of callus and the technology of regeneration plant were studied using the stem fragment with bud, tender embryo and cotyledon as initial explants. For the stem fragment, the induced rate is up to 83.33% on MS medium supplemented with 6-BA(3.0 mg·L^(-1)) and NAA(1.0 mg·L^(-1)), and many arbusive buds could be get on the same medium. Callus could be induced effectively from the tender embryo and cotyledon on the medium supplemented with 2,4-D(2.0 mg·L^(-1)) and KT(1.0 mg·L^(-1)). For regeneration of adventitious bud from the callus, medium supplemented with 6-BA(3.0 mg·L^(-1)) and NAA(0.05 mg·L^(-1)), or 6-BA(2.5 mg·L^(-1)) and IAA(1.5 mg·L^(-1)) was appropriate, and the regeneration frequency is above 90%. The bud could grow into a seedling on the MS medium supplemented with additional NAA(7 mg·L^(-1)), and the rooting rate could reach up to 93%.