摘要
综述了应用于污染土壤微生物多样性分析的各种分子生物学方法 ,包括DNA中mol%G +C丰度的分析、核酸杂交技术、DNA复性动力学研究及基于PCR技术的DNA指纹图谱分析等方法。
Several molecular biotechniques which have been used for study on microbial diversity in polluted soils were reviewed. These approaches include analysis of mol% (G+C) abundance of DNA,nucliec acid hybridisation,DNA reassociation kenitics and DNA fingerprints pattern based on PCR technique and so on. Both of the advantages and limitations of these approaches were also discussed.
出处
《土壤学报》
CAS
CSCD
北大核心
2004年第4期612-617,共6页
Acta Pedologica Sinica
基金
沈阳环境工程重点实验室
中国科学院知识创新工程重大项目(KZCX2401)资助
关键词
分子生物学方法
土壤污染
微生物多样性
DNA复性动力学
核酸杂交
Polluted soil
Microbial diversity
16S rRNA gene sequence
Nucliec acid hybridization
DNA reassociation kenitics
mol% G+C
