A3α肽聚糖对牙鲆不同组织中超氧化物歧化酶及磷酸酶活性的影响

Effect of A3α peptidoglycan (PG) on SOD, ACP, and AKP activities in different tissues of Japanese flounder Paralichthys olivaceus

研究了口服不同剂量的A3α肽聚糖 (A3α -PG)对牙鲆 (Paralichthysolivaceus)体表粘液和组织中超氧化物歧化酶(Superoxidedismutase,SOD)、酸性磷酸酶 (Acidphosphatase,ACP)及碱性磷酸酶 (Alkalinephosphatase,AKP)活力的影响。饲料中A3α -PG添加量分别为 0 (对照 ) ,0 .0 5 % ,0 .10 % ,0 .2 0 % ,0 .4 0 % ,0 .80 %和 1.6 0 % (质量比 ) ,饲喂天数为 4 0d。结果表明 ,饲料中未添加A3α -PG时 ,牙鲆体表粘液、肾脏、肝脏和肌肉中SOD、ACP及AKP活性各异 ,其中SOD活性从高到低依次为粘液、肝脏、肌肉、肾脏 ;ACP活性大小依次为肝脏、肾脏、肌肉、粘液 ;AKP活性大小依次为肾脏、肝脏、肌肉 ,粘液中则检测不到AKP。饲料中添加A3α -PG后 ,实验组与对照组相比 ,各组织中SOD活性变化不大 ,未见显著性差异 (P>0 .0 5 )。ACP活性 ,肌肉与粘液中未见显著提高 ;而在肝脏中 0 .0 5 %和 0 .4 0 % (质量比 )剂量组能显著提高其ACP活性 ,与对照差异显著 (P <0 .0 5 ) ;在肾脏中 ,0 .2 0 % (质量比 )剂量组使其ACP活力得以显著提高 (P <0 .0 5 )。AKP活性呈现不同组织变化趋势各异 ,肌肉中没有明显提高 ,而在肾脏与肝脏中 ,除两个低剂量组 (0 .0 5 %和 0 .10 % ) (质量比 )未能使AKP活力得以显著提高外 ,其他剂量组?

The A3α peptidoglycan (A3α-PG) was prepared from Bifidobacterium thermophilum.Peptidoglycan is a long-chain polysaccharides and is a good stimulator of non-specific defence mechanism in animals including fish. The effect of A3α-PG on superoxide dismutase (SOD), acid phosphatase (ACP) and alkaline phosphatase (AKP) activities in different tissues of Japanese flounder Paralichthys olivaceus, an improtant seawater fish used in intensive framing, was studied by supplying various A3α-PG levels in diets. The purpose of this study was to determine if peptidoglycan administration could enhance the activity of antioxidant and hydrolytic enzyme. The experimental dietary levels of A3α-PG were 0 (control), 0.05%, 0.10%, (0.20%,) 0.40%, 0.80% and 1.60% (w/w) respectively. After 40 days feeding, the results indicated that the activities of SOD were mucus>liver>muscle>kidney, the activities of ACP liver>kidney>muscle>mucus and the activities of AKP kidney>liver>muscle in contral (without supplying A3α-PG). The activity of AKP in mucus were not defected. Compared with control, the SOD activity was unaffected in different tissues at different A3α-PG levels. The activities of ACP were not observed significant increases in muscle and mucus, while in liver, when A3α-PG supplement levels were 0.05% and 0.40% (w/w), the activities of ACP significantly increased (P<0.05). The same phenomenon was observed in kidney when A3α-PG supplement level was 0.20% (w/w). The activities of AKP, there were various in the different tissues. The AKP activity in muscle tissue was not enhanced obviously. While those of in kidney and liver were significantly promoted with A3α-PG supplement increasing in the range from 0.20% to 1.60% (w/w) (P<0.05 or P<(0.01).) In conclusion, as an immunopolysaccharide, A3α-PG can regulate the antioxidant enzyme and hydrolytic enzyme, and improve the non-specific immunity. For the immunomodulatory effect of A3α-PG. Taking the economy and efficiency into account, it is suggested that the feasible dosage should be 0.20%-(0.40%) (w/w).