大鼠严重烫伤后睾丸病理变化的实验研究

Pathological changes of the testes after scalding in rats

对大鼠体表30％、Ⅲ°烫伤后30d内睾丸病理变化的动态观察表明:(1)生精细胞、支持细胞、曲细精管界膜及间质细胞均有不同程度的损伤,生精细胞的病变以精母细胞及精细胞为重,精原细胞无明显损伤;伤后30d生精上皮形态基本恢复正常。(2)间质细胞内3β—HSD活性迅速降低,伤后30d仍保持较低水平。(3)烫伤后血清中睾酮迅速下降,伤后30d仍保持较低水平;而血清LH则无明显变化。作者认为曲细精管界膜及支持细胞的损伤,特别是后者,改变了生精细胞生长、发育的微环境而可能引起或加重生精细胞的损伤。血清睾酮下降的机理,可能与烫伤后糖皮质激素升高有关。

In the 30-day period after the rats were inflicted with 30% TBSA full thickness scalding, the main pathological changes of the testes were various degrees of degeneration and necrosis of the spermatogenic cells, Sertoli's cells, lamina propria of the seminiferous tubules and Leydig's cells. The damages on spermatogenic cells, spermatocytes and spermatids were quite severe while spermatogonial cells exhibited little damages. On the 30th day after scalding, the spermatogenic cells restored their normal morphology. The activity of 3β-hydroxy steroid dehydrogenase was rapidly reduced and remained at a relatively low level on the 30th day after scalding. The serum level of testosterone was decreased and remained at a comparatively low level on the 30th day after scalding. No significant changes of serum luteinizing hormone was observed.It is believed that the damages on the lamina propria of the seminiferous tubules and Sertoli's cells espercially the latter could change the microenvironment of the development and differentiation of the spermatogenic cells and the decrease of serum testosterone might result from the increase of glucocorticoids after scalding.