小柴胡汤和柴胡—黄芩含药血清对CCl_4损伤肝细胞的影响

Effects of XCHT and bupleurum root-scutellaria root on hepatocyte injury model induced by CCl_4 in vitro

目的 建立CCl4肝细胞损伤模型 ,考察小柴胡汤及柴胡—黄芩配方含药血清对CCl4损伤肝细胞的保护作用。方法 制备小柴胡汤及柴胡—黄芩配方的大鼠含药血清 ,体外10mol·L-1CCl4诱导肝细胞损伤模型 ,观察体积分数为 0 1的含药血清对损伤肝细胞增殖率、GPT、细胞形态学以及凋亡率的影响。结果 与正常组比较 ,模型组肝细胞增殖率降低 (P <0 0 1) ,细胞培养上清液中GPT含量升高 (P <0 0 1) ;与模型组比较 ,小柴胡汤及柴胡—黄芩配方组的肝细胞增殖率提高 (P <0 0 1) ,细胞培养上清液中GPT含量降低 (P <0 0 1)。倒置显微镜和Giemsa染色观察到模型组肝细胞存在着明显的坏死和凋亡 ,小柴胡汤及柴胡—黄芩配方的凋亡细胞明显减少。与正常组比较 ,CCL4模型组细胞凋亡增高 (P <0 0 1) ;与模型组比较 ,柴芩组和小柴胡汤全方组的细胞凋亡率降低 (P <0 0 1)。结论 小柴胡汤及柴胡—黄芩配方含药血清对体外CCl4诱导的肝细胞损伤有保护作用。CCl4引起的肝损伤同时存在着明显的凋亡和坏死 ,小柴胡汤及柴胡 -黄芩配方可能具有抗CCl4诱导的肝细胞凋亡的作用。

AIM To explore the effects of serum containing XCHT and bupleurum root-scutellaria root on hepatocyte injury model. METHODS The hepatocyte injury model was induced by CCl 4(10 mol·L -1). The injured hepatocytes were incubated with CCl 4 and serum containing XCHT or bupleurum root-scutellaria root for 24 h. Then the hepatocyte proliferation rate and GPT concentration in cell culture medium were analyzed. The cells were observed by microscope and Giemsa staining. The apoptosis rate was measured by flow cytometry. RESULTS The serum containing XCHT and bupleurum root-scutellaria root could improve the hepatocyte proliferation rate that was injured by CCl 4 significantly (P<0.01) and decrease GPT concentration in cell culture medium(P<0.01). There were obvious necrosis and apoptosis in the model group cells observed under the convert microscope and Giemsa staining. CCl 4 induced the apoptosis of model group cells(P<0.01). The original recipe of XCHT and bupleurum root-scutellaria root resisted the apoptosis induced by CCl 4(P<0.01). CONCLUSION The serum containing XCHT and bupleurum root-scutellaria root could protect injured hepatocyte induced by CCl 4 in vitro. CCl 4 obviously induces apoptosis and necrosis of the hepatocyte. XCHT and bupleurum root-scutellaria root are of the effects of resisting hepatocye apoptosis induced by CCl 4.