摘要
利用SephadexG 2 0 0和SephadexG 1 0 0 2次凝胶过滤层析的方法 ,从猪卵泡液中初步分离得到抑制素及其α亚基的粗提物。通过免疫印迹的方法检测 。
Crude inhibin was abstracted from the porcine follicular fluid by Gel permeation chromatography with Sephadex G-200 and Sephadex G-100 and identificated by western blot with monoantibody of inhibin α subunit. The result showed the existence of inhibin α subunit in the abstracts.
出处
《畜牧与兽医》
北大核心
2004年第7期12-13,共2页
Animal Husbandry & Veterinary Medicine
基金
教育部博士基金资助项目 (2 0 0 1 0 30 70 0 7)
关键词
抑制素
凝胶过滤层析
免疫印迹
inhibin
gel permeation chromatography
western blot
