氧化电位水对消化内镜消毒效果的研究

The effectiveness of electrolyzed oxidizing water in disinfections of endoscopes

目的 检测氧化电位水(electrolyzed oxidizing water,EOW)对消化内镜的消毒效果并与2％戊二醛相比较。方法 (1)对一般细菌的消毒效果:顺次抽取检查患者后的消化内镜,EOW组和2％戊二醛组均分别浸泡消毒1、3、5 min,每时间组均为30例,进行细菌培养。(2)对幽门螺杆菌(Hp)的消毒效果:顺次抽取快速尿素酶试验阳性者使用过的以及Hp人为污染后的内镜,2种消毒剂按消毒时间分组同上,PCR法检测Hp残留。(3)对乙肝病毒(HBV)的消毒效果:用HBV阳性血清稀释液污染内镜,2种消毒剂分别作用1、3、5、10 min,每时间组30例,PCR法检测HBV残留。结果(1)一般细菌:消毒1、3、5 min后,EOW组细菌培养均为阴性,2％戊二醛组分别有阳性5例、4例、1例。(2)Hp:EOW组作用1 min后2例阳性,3、5 min后的均为阴性;2％戊二醛作用1、3、5 min后分别有3例、4例、4例阳性。(3)HBV:EOW组作用1、3、5、10 min各组均为阴性;2％戊二醛组作用后各时间组分别有2例、5例、4例、5例阳性。结论 EOW对一般细菌、Hp、HBV等有较强的杀灭作用,较2％戊二醛消毒力强、快速,可推荐为临床内镜消毒。

Objective To observe the effectiveness of electrolyzed oxidizing water(EOW) in disinfections of endoscopes and compared with that of 2% glutaraldehyde. Methods (1) Disinfections of bacteri-a:Specimens were obtained immediately from endoscopes after being used, disinfected and washed out for bacterial culture. Endoscopes were immersed in either EOW or 2% glutaraldehyde for 1 , 3 and 5 minutes. Each time group consisted of 30 cases. (2 ) Effectiveness on disinfections of H. Pylori: endoscopes either from patients with H. Pylori infection or artificially contaminated with H. Pylori were washed out and exposed for 1 , 3 and 5 minutes in each of the disinfectants. PCR was used to detect DNA of H. Pylor. (3) Effectiveness of disinfections of HBV : Endoscopes were contaminated with HBV-positive serum, and the exposure time to both disinfectants were 1,3,5 and 10 minutes respectively. Thirty samples were studied in each time groups. Results ( 1 ) After manual cleaning and disinfections for 1, 3 and 5min in EOW, no vegetative bacteria were found. In 2% glutaraldehyde processing group, 5/30, 4/30 and 1/30 specimens remained bacteria positive, respectively. (2)There was no positive specimens found after 3 and 5 minutes' exposure in EOW in the group of H. Pylori, but 2/30 positive specimens were found in the 1 minute group. While 3/30, 4/30 and 4/30 positive specimens were found after exposure in 2% glutaraldehyde. (3) In the group of HBV, there is no positive case found after exposure in EOW, while in 2% glutaraldehyde groups, there were 2,5 , 4 and 5 cases remained positive in 1 ,3,5 and 10 minutes group respectively. Conclusion EOW is superior to 2% glutaraldehyde in disinfections of Hp or HVB with high-speed and thoroughness, and worthy to be recommended in disinfections of endoscopes.