6-BA和NAA对袋鼠花组织培养特性的影响

Effect of 6-BA and NAA on the tissue culture of Anigozangthos

研究了不同质量浓度的6-BA和NAA对袋鼠花侧芽组织培养特性的影响。结果表明,6-BA与NAA配合使用能有效促进袋鼠花侧芽愈伤组织的形成和愈伤组织不定芽的发生。促进愈伤组织形成的最佳激素配比为MS+6-BA1.0mg/L+NAA0.5mg/L;6-BA对袋鼠花侧芽愈伤组织不定芽的发生有显著的促进作用,在低质量浓度NAA条件下,随着培养基中6-BA质量浓度的增加,促进效应更加明显,适合袋鼠花侧芽愈伤组织不定芽形成的最佳激素处理为MS+NAA0.2mg/L+6-BA2.0mg/L;附加一定质量浓度NAA的1/2MS培养基,能显著促进袋鼠花不定芽根的形成,其最佳培养基为1/2MS+0.1mg/LNAA。

The cultural characteristic of Anigozangthos bud in vitro was studied using madia containing 6-BA and NAA.The results showed that the optimum concentration combination of 6-BA with NAA in the media could significiantly promote dedifferentiation of Anigozangthos bud tissue and redifferentiation of Anigozangthos calli.The best medium for callus formation of Anigozangthos bud tissue was MS medium with 6-BA 1.0 mg/L,NAA 0.5 mg/L.It was found that 6-BA could promote redifferentiation of Anigozangthos callus significiantly,the frequency of Anigozangthos callus adventitious shoot production was increased with increasing concentration of 6-BA in medium containing low concentration NAA.The best medium for Anigozangthos callus adventitious shoot production was MS medium with 6-BA 2.0 ( mg/L),NAA 0.2 mg/L.The results also showed that NAA could promote adventitious root formation of Anigozangthos adventitious shoot,the best medium for root formation of Anigozangthos adventitious shoot was 1/2 MS medium with 0.1 mg/L NAA.