2型糖尿病大鼠肾脏病变中血小板CD62P的变化及意义

The Change of Platelet CD62P in Different Stages of Type 2 Diabetes Mellitus Rats and Its Relationship with Renal Disease

目的探讨血小板CD62P在2型糖尿病大鼠肾脏病变不同阶段的变化及意义。方法Wis tar大鼠给予高脂高糖饲料喂养4周形成胰岛素抵抗后 ,腹腔注射小剂量链脲佐菌素 (STZ ,30mg·kg-1)诱导形成2型糖尿病模型 ,分别在胰岛素抵抗期、糖尿病成模12周、22周检测各组大鼠血小板CD62P的表达、尿白蛋白排泄率及肾脏形态学改变。结果①胰岛素抵抗期 ,血小板CD62P已有增高趋势 ,但是与正常对照组相比统计学上无显著差异(P>0.05)。此时肾脏形态学、尿白蛋白排泄率无明显变化 ;②糖尿病成模12周时 ,血小板CD62P的表达较对照组显著增高 (P<0.001) ,尿白蛋白排泄率无明显变化 ,光镜下肾小球系膜基质明显增多 ;③在糖尿病成模22周时 ,血小板CD62P的表达与对照组相比无明显差异(P>0.05) ,此时尿白蛋白排泄率较对照组显著增高(P<0.01),肾小球形态学改变表现为弥漫性硬化和纤维化。结论2型糖尿病大鼠肾脏病变过程中 ,血小板CD62P的表达可能与病变的不同阶段有关。

Objective To study the change of platelets CD62P in the different stages of diabetes mellitus rats and its significance in the process of diabetic nephropathy(DN).Methods Wistar rats were fed on a diet enˉriched in glucose and fat for4weeks to induce insulin resistance(IR),then injected intraperitoneally with lowdose streptozotocin(30mg.kg -1 once a week)to induce type2diabetes.In the insulin resistance stage,in the12th week after developing diabetes and the22th week platelets CD62P were determined by flow cytometory(FCM),uriˉnary albumin excretion(UAE)was measured by RIA,and the renal morphologic changes were observed under light microscope.Results In the IR stage,platelets CD62P had a trend to increase but it was not significant(P>0.05),meanwile,UAE and renal morphology were not markedly different compared with controls.In the12th week after developing diabetes the expressions of platelets CD62P were significantly enhanced compared with the control group(P<0.001),and UAE were not significantly different,but the glomerular mesangium matrix had obˉviously increased.In the22th week platelets CD62P were not significantly different compared with control group(P>0.05),but UAE in diabetic group was significantly higher than that in the other group(P<0.01)and the renal morphologic changes became more serious.Conclusions The expressions of platelets CD62P in the proˉgression of diabetes rats may be associated with different stages of DN.