烧骨DNA检验技术的研究

Study of DNA identification in burned bones

目的解决陈旧性骨骼和烧骨DNA检验难题。方法研究建立了CTAB法裂解提取DNA,再用磁珠纯化得到的DNA提取液进行STR复合扩增检验。结果实验结果及实际检案显示研究所建立的骨DNA提取方法能较好地去除DNA扩增抑制物,得到高质量的DNA模板。结论本研究所建立的烧骨DNA检验方法其识别率为10×10-12,达到个人同一认定的目的,在解决实际工作中杀人焚尸案、火灾、爆炸等恶性案件和事故中有重要的作用。

Objective For the purpose of solving a problems of DNA testing of burned bones.Methods We present a novel strategy to obtain DNA from burned bones based on the use of cetyltrimethylammoni-um bromide(CTAB)lysis buffer and isoamyl alcohol-chlorophorm extraction with subsequent DNA purifi-cation using the DNA IQTMSystem.Results The methods were found to be effective in removing the PCR inhibitors from the burned bone.Then the extracted DNA was successfully genotyped by using the flores-cence labeling STR multiplex method.Conclusion The results of this research will assist forensic scien-tists in the identification of DNA from victims whose bodies underwent significant trauma or burning,pre-cluding the utilization of traditional forensic DNA identification techniques.