赤芍水提物对四氯化碳致肝损伤大鼠的保护作用

Effect of Aqueous Extract of Radix Paeoniae Rubra against Carbon Tetrachloride Induced Liver Fibrosis in Rats

目的 :探讨赤芍水提取物对四氯化碳 (CCl4 )中毒性肝纤维化大鼠的治疗作用。方法 :复制大鼠CCl4 肝纤维化模型 ,以马洛替酯为阳性对照 ,采用光镜观察组织学改变 ,测定血清丙氨酸氨基转移酶 (ALT)、天门冬氨酸氨基转移酶 (AST)、一氧化氮 (NO)、透明质酸 (HA)、层粘蛋白 (LN) ,肝组织羟脯氨酸 (Hyp)、丙二醛 (MDA)以反映肝细胞损伤及肝纤维化的程度。结果 :赤芍水提物能降低实验性肝纤维化大鼠血清中升高的ALT、AST、NO、HA、LN水平和肝组织中过高的Hyp、MDA的含量。病理组织学检查亦表明 ,赤芍水提物明显改善实验性肝纤维化。结论 :赤芍水提物对实验性肝纤维化具有治疗作用。

Objective:To probe into the therapeutic effect of the aqueous extract of radix paeoniae rubra in the treatment of liver fibrosis induced in rats by carbon tetrachloride (CCl 4). Methods:Model of liver fibrosis in rats was induced by a series of subcutaneous injections of CCl 4. The animals of the treatment group were given each 20,40 or 80 mg·kg -1 of radix paeoniae rubra q.d. while those of the positive control group were given each 90 mg·kg -1 of malotilate q.d.. Drugs in both groups were administered by gastrogavage,and the course of treatment lasted 12 consecutive weeks in both groups. The liver histology was studied with light microscopy. Levels of serum alanine aminotransferase(ALT),aspartate aminotransferase (AST),nitric oxide (NO),hyaluronic acid(HA) and laminine (LN) as wells as contents of hydroxyproline (Hyp),malonyldialdehyde (MDA) of the liver were determined to mirror the extent of liver injury and hepatic fibrosis. Results:The elevated serum levels of ALT,AST,NO,HA and LN as well as the increased content of Hyp and MDA in the liver tissue were significantly brought down by radix paeoniae rubra. Pathological examination also demonstrated that radix paeoniae rubra could strikingly alleviate the experimental hepatic fibrosis. Conclusion:Aqueous extract of radix paeoniae rubra was shown to be effective in the treatment of experimental liver fibrosis in rats.