高效液相色谱指纹图谱在中药芦根上的应用

Application of High Performance Liquid Chromatographic Fingerprint to Phragmites Communis Trin., a Traditional Chinese Medicine

利用现代分离分析技术如高效液相色谱 (HPLC)与气相色谱 (GC) ,能够获得中草药的色谱指纹图谱。本实验报道了高效液相指纹图谱应用于快速检测中草药提取物以及应用于研究、开发与生产中的质量控制体系中 ,利用HPLC建立芦根的色谱指纹图谱。实验条件为反相C1 8柱 ,流速为 1 .0mL/min ,紫外检测器 (λ=2 30nm)。通过分析比较色谱指纹图谱中的相对保留值α以及相对面积Sr 对不同产地的芦根进行对比研究。这种基于HPLC的分析策略为中草药的精确鉴定和质量控制提供了有效的信息 。

This paper describes an analytical strategy by using high performance liquid chromatography (HPLC) to rapidly analyse the mixture extracted from the traditional Chinese medicine and support the quality control for research, development and manufacturing. This strategy utilizes HPLC involves a RP-C-18 column, an ultraviolet detector at 230 nm to establish the chromatographic fingerprint of Phragmites communis Trin.. By using the relative retention value alpha and the relative area S-r in chromatographic fingerprint, the comparative studies for different resources of Phragmites communis Trin. were achieved. This HPLC-based strategy provides effective information for the accurate identification and quality control of materia medica for traditional Chinese medicine, and is a development for the application of HPLC to the sample analysis of complex components.