AFLP技术用于巴马小型猪遗传多样性的研究

Study on Genetic Diversity of Bama Miniature Pigs by AFLP Technique

目的 评价巴马小型猪的遗传多样性。方法 用与接头对应的 10条AFLP引物 ,扩增经PstⅠ酶切且与接头连接的 17头巴马小型猪的基因组DNA ,用从Internet网上下载的软件RAPDistancepackageversion 1 0 4对结果进行分析。结果 共获得 10 4个AFLP标记 ,单引物得到的标记数在 3～ 2 0个之间 ,巴马小型猪群体内的相似系数为 0 90 3(0 791～ 0 982 )。其多态性位点的比率为 2 9 8%;总的等位基因数为 136个 ,平均每个位点的等位基因数为 1 31个 ,基因型数为 119,平均每个位点的基因型数为 1 4 1。结论 该实验为评价巴马小型猪遗传多样性的多寡提供了相关的参数 ,准确评价尚待和普通猪对比研究后确定。

Objective To determine genetic diversity of Bama miniature pigs. Methods 10 AFLP primers corresponding adapter were used to amplify genomic DNAs of Bama miniature pigs. The amplified fragments were analysed by the RAPD Distance Package version 1.04. Results 104 AFLP markers were obtained and the markers of individual primer were between 3-20, with the similary index of 0.903(0.791-0.982). The proportion of polymorphic loci was 29.8%. Total number of alleles was 136, average number of alleles 1.31. The number of genotype was 119, average number of genotype 1.14. Conclusion Useful parameters were provided for monitoring genetic stability of Bama miniature pigs.