鸡PPARs基因组织表达特性的研究

Characterization of Tissue Expression of Peroxisome Proliferator Activated Receptors in the Chicken

以 8周龄ArberAcres(AA)肉鸡为研究材料 ,采用RT PCR和Northernblot方法对鸡PPAR基因组织表达特点进行了研究。RT PCR半定量检测显示 ,在检测的 10种组织中除胸部肌肉组织外 ,PPAR α基因在其他 9种组织中都表达 ,其中较高表达于脑、肺脏、肾脏、心脏和小肠 ,中等程度表达于胃、肝脏和脂肪 ,较低表达于脾脏。PPAR γ基因除了在肝脏和肌肉中没有检测到外 ,在其他 8种组织都有表达 ,其中高表达于脂肪 ,其次是脑和肾脏 ,低量表达于脾脏、心脏、肺脏、胃和小肠。Northernblot检测显示 ,PPAR α基因在心脏、肝脏、肾脏和胃这 4种组织表达 ,其中在肝脏杂交信号最强 ;PPAR γ基因只在脂肪和肾脏表达 ,其中在脂肪组织有强的杂交信号。以上结果表明 ,鸡PPARs基因的组织表达特点同啮齿动物和人基本一致 ,但也有其自身的特殊性 ,即PPAR α基因不在肌肉组织中表达 ,PPAR γ基因在肾脏中有高表达。PPAR基因与鸡的多种组织的生长和发育有关。

The peroxisome proliferator activated receptor (PPAR) is the member of super family of nuclear receptors.A great number of researches in rodent and human have showed that PPARs are involved in the lipids metabolism,especially in regulating the microsome,expression of genes coding the various enzymes during the β-oxidation of peroxisome and the differentiation of adipose cell.The PPARs are one of the important gene clusters that are viewed as the objective genes correlated with the lipids metabolism and corresponding diseases.Great differences in lipids metabolism are existed between the poultry and mammals.The mechanism of the lipids metabolism in poultry is not completely understood.The current study was designed to investigate the expression characterization of PPAR genes in chicken in order to understand the effects of PPAR gene on adipose and other tissues.The eight weeks age Arber Acres broilers were used and quantitative RT-PCR and Northern blot were developed to analyze the expression characterization of PPAR-α and PPAR-γ gene in heart,liver,spleen,lung,kidney,stomach,intestine,brain,muscle and adipose tissues.Results of the quantitative RT-PCR showed that the expression level of PPAR-α gene was higher in brain,lung,kidney,heart and intestine,medium in stomach,liver and adipose,and lower in spleen.But did no expression was detected in breast muscle.The expression level of PPAR-γ gene was higher in adipose,medium in brain and kidney,lower in spleen,heart,lung,stomach and intestine,but no expression was detected in liver and breast muscle.Northern blot results showed that PPAR-α gene expressed in heart,liver,kidney and stomach,and the intensity of hybridization signal was the greatest in liver tissue;PPAR-γ gene only expressed in adipose and kidney tissues,and the intensity of hybridization signal was greater in adipose tissue.The present study results showed that the tissue expression characterization of PPARs gene in chicken was almost consistent with that in rodent and human,but, in chicken, PPAR-α did not expressed in breast muscle and PPAR-γ gene expressed in kidney.The results of current research indicated simultaneously that the PPAR genes were related with the growth and development of various tissues and organs.