20(S)-人参皂苷Rg3抗B16黑色素瘤转移的作用

Inhibitory effect of 20(S)-ginsenoside Rg_3 on B16 melanoma metastasis

目的 :探讨 2 0 (S) -人参皂苷 Rg3抗 B16黑色素瘤转移作用及其机制。方法 :采用 B16黑色素瘤自发肺转移和人工肺转移模型观察 Rg3抗肿瘤转移作用 ,观察 Rg3对 B16黑色素瘤诱导的肿瘤新生血管的形成及B16黑色素瘤细胞自身侵袭能力的影响。结果 :在 B16黑色素瘤自发肺转移和人工肺转移实验中 ,Rg3组 C5 7BL/6 N小鼠肺部转移结节数显著减少 ,且 Rg3组肿瘤周围的血管数也明显减少 ;Rg3作用后的 B16黑色素瘤细胞侵袭人工基底膜能力明显下降。结论 :Rg3可明显抑制 B16黑色素瘤肺转移 ,该作用可能与 Rg3抑制肿瘤新生血管形成以及降低 B16黑色素瘤细胞的侵袭能力有关。

Objective To study the inhibitory effect of 20(S)-ginsenoside Rg 3 on B16 melanoma metastasis and its mechanism. Methods The spontaneous and experimental lung metastasis models of B16 melanoma were used to investigate the inhibitory effects of Rg 3 on tumor metastasis. The assay of tumor-induced angiogenesis was used to observe the effect of ginsenoside Rg 3 on B16 melanoma angiogenesis. The effect of Rg 3 on invasive ability of B16 cells in vitro was detected by Boyden chamber. Results In the spontaneous and experimental lung metastasis models of B16 melanoma, the number of tumor colonies in lung in each Rg 3 group was lower than that in control group. The number of vessels oriented toward the tumor mass and the invasive ability of B16 cells decreased obviously after treated with Rg 3. Conclusion 20(S)-ginsenoside Rg 3 can obviously inhibit the lung metastasis of B16 melanoma. The inhibitory effect partially dues to its inhibiting angiogenesis induced by B16 melanoma and decreasing the invasive ability of B16 cells.