甘草酸对血清和组织胺诱发的大鼠气道平滑肌细胞增生的影响

Effects of glycyrrhizin on the proliferation of ASMC induced by fetal calf or histamine in rats

目的 观察甘草酸对血清和组织胺诱发的大鼠气道平滑肌细胞 (ASMC)增生的影响。方法 MTT法、细胞计数法和流式细胞术检测体外培养的ASMC的光密度、细胞数和细胞周期。结果 ①在含 10 0g·L-1FCS的培养液中加入 6×10 -5mol·L-1的甘草酸可使A570 的增速增加 ,而加入 384×10 -5mol·L-1和 15 36× 10 -5mol·L-1却使A570 的增速减慢 ;在含 10 -2 mol·L-1组织胺的培养液中加入甘草酸使A5 70的增速减慢 ,浓度越大作用越明显。②在含 10 0g·L-1FCS的培养液中加入 6× 10 -5mol·L-1的甘草酸可促进细胞增生 ,而加入 384× 10 -5mol·L-1和 15 36× 10 -5mol·L-1则抑制细胞增生 ;在含 10 -2 mol·L-1组织胺的培养液中加入甘草酸 ,细胞增生受到抑制 ,浓度越大作用越明显。③在 10 0g·L-1FCS或 10 -2 mol·L-1的组织胺中培养 96h ,随着甘草酸浓度的增加 ,G1期细胞数增多 ,S期和G2 +M期细胞数减少。结论 ①对FCS刺激引起的ASMC增生 ,低浓度甘草酸有促进作用 ,高浓度有抑制作用。②对组织胺刺激引起的ASMC增生 。

Aim To investigate the effects of glycyrrhizin on the proliferation of ASMC stimulated by fetal calf or histamine in rats. Methods Cell culture of rat ASMC, MTT assay, flow cytometry and cell growth counts were used in this study. Results ①In the culture medium containing 100g·L -1 fetal calf serum, Glycyrrhizin at low concentration(6×10 -5 mol·L -1 ) stimulated the increase of A 570 in ASMC. This effect descended with increasing glycyrrhizin concentration and changed to be inhibitory at high concentration (from 384×10 -5 mol·L -1 to 1 536 ×10 -5 mol·L -1 ). In the culture medium containing 10 -2 mol·L -1 histamine, Glycyrrhizin at both low and high concentration inhibited the increase of A 570 in ASMC. ②In the culture medium containing 100 g·L -1 fetal calf, Glycyrrhizin at low concentration(6×10 -5 mol·L -1 ) stimulated the proliferation of ASMC. This effect descended with increasing glycyrrhizin concentration and changed to be inhibitory at high concentration (from 384×10 -5 mol·L -1 to 1 536 ×10 -5 mol·L -1 ). In the culture medium containing 1 g·L -1 histamine, Glycyrrhizin at both low and high concentration inhibited the proliferation of ASMC. ③In the culture medium containing 100 g·L -1 fetal calf or 10 -2 mol·L -1 histamine, with increasing glycyrrhizin concentration, the cell count in G 1 phase increase, the cell count in G 2 and M phase decrease. Conclusion ①Glycyrrhizin accelerated the proliferation of ASMC stimulated by fetal calf at low concentration, inhibited the proliferation at high concentration. ②Glycyrrhizin inhibited the proliferation of ASMC stimulated by histamine at both low concentration and high concentration.