新生儿微量血血清胆红素测定的影响因素分析

Study of the effect factors on the detection of neonate serum minute quantities bilirubin diglucuronide

目的探讨影响新生儿微量血血清胆红素测定结果的因素。方法对 38例黄疸患儿同时采集微量血 3份 ,即刻离心测定微量血清胆红素后 ,甲份置于室温下不避光 ,乙份置于室温下避光 ,丙份置于 4℃左右的冰箱保存 ,待 1h、2h、2 4h及 4 8h再次测定微量血胆红素。结果置于室温下不避光组 2 4h、4 8h与采血即刻所测微量血清胆红素结果的下降率分别为 31 9%和 4 4 5 % ;室温下避光组 2 4h、4 8h与采血即刻所测微量血清胆红素结果的下降率分别为 :7 6 %、12 7% ;冰箱组 2 4h、4 8h与采血即刻所测微量血清胆红素结果的下降率分别为 2 6 %和 3 6 %。结论自然光线照射后各时相所测微量血清胆红素值水平明显降低 ,而在冰箱内低温避光保存后各时相所测微量血清胆红素值水平下降不明显。因此 。

Objective To study the effect factors on the detection of neonate serum minute quantities bilirubin diglucuronide Method 30 cases of neonates with jaundice were selected, and three blood samples were taken from each neonate. Blood samples were divided into A, B and C group. Minute quantities bilirubin diglucuronide were detected immediately after collecting and centrifuging, then Group A sample was stored under without photophobic and room temperature location. Group B sample was stored under photophobic and room temperature location. Group C sample was stored about 4 ℃ in refrigerator. Bilirubin diglucuronide were detected again 1h, 2h, 24h, and 48 h after storage. Result Compared with result detected before storage, 24h and 48 h after storage, The reduce rate of bilirubin diglucuronide in group A were 31.9% and 44.5%. 7.6% and 12.7% for group B. 2.6% and 3.6% for group C. Conclusion the natural light lead to the remarkably reduce of serum bilirubin diglucuronide. It is suggested that the undetected blood samples should be stored in refrigerator and photophobic.