AEC对金针菇菌丝生长和粉孢子萌发的抑制作用

Effect of AEC on the Mycelial Growth and Oidium Germination of Flammulina velutipes

在PDA培养基和基本培养基中添加不同浓度的赖氨酸结构类似物S (2 氨基乙基 ) 2′半胱氨酸 (AEC) ,观察其对金针菇IM0 2单核菌丝和金白 1号双核菌丝生长及其粉孢子萌发的抑制作用。结果表明 ,AEC对金针菇IM0 2单核菌丝和金白 1号双核菌丝生长的抑制作用显著不同。当PDA培养基中AEC浓度为 2 0 0 0mg/L时 ,对金针菇IM0 2单核菌丝生长速度的抑制率为5 6 .4 % ;金白 1号双核菌丝对AEC较敏感 ,AEC浓度为 6 2 .5mg/L时 ,金白 1号双核菌丝生长完全受到抑制。当基本培养基中AEC浓度为 12 0 0mg/L时 ,对IM 0 2单核菌丝生长速度的抑制率可达 82 .5 % ;而当培养基中AEC浓度为 37.5mg/L时 ,金白 1号双核菌丝生长完全受到抑制。当PDA培养基中AEC浓度为 2 0 0 0mg/L时 ,10d内未见金针菇IM 0 2单核菌丝和金白 1号双核菌丝产生的粉孢子萌发。本试验为金针菇高赖氨酸良种的选育奠定了基础。

The inhibition effect of lysine analogue( S-2-aminoethyl-L-cysteine,AEC)with different concentrations on the mycelial growth and oidium germination of Flammulina velutipes was observed in PDA medium and minimal medium. The experiment results showed that the inhibition effect of AEC on the growth of Flammulina velutipes IM02 mononuclear mycelium and Jingbai No.1 binuclear mycelium was significantly different. In PDA medium, when AEC concentration was 2000mg/L,the inhibition rate of AEC on the growth of Flammulina velutipes IM02 mononuclear mycelium was 56.4% ;when AEC concentration was 62.5mg/L, the growth of Flammulina velutipes Jingbai No.1 binuclear mycelium was completely inhibited. In minimal medium, when AEC concentration was 1200mg/L, the inhibition rate of AEC on the growth of Flammulina velutipes IM02 mononuclear mycelium was 82.5%; when AEC concentration was 37.5mg/L,the growth of Flammulina velutipes Jingbai No.1 binuclear mycelium was completely inhibited. When AEC concentration was 2000mg/L in PDA medium,the oidium germination of both IM02 mononuclear mycelium and Jingbai No.1 binuclear mycelium of Flammulina velutipes was completely inhibited within 10 days.The experiment results of our research will lay a foundation for breeding lysine-rich strain of Flammulina velutipes.