摘要
通过 96h的毒性实验 ,研究了普通小球藻对 3种不同作用机制农田常用除草剂嗪草酮、骠马和甲草胺的敏感性 .结果表明 ,在实验条件下 ,嗪草酮、甲草胺对普通小球藻的毒性随时间的推移有加重趋势 ,并呈现很好的剂量效应关系 ;最高抑制生长浓度 (嗪草酮 0 2 4mg·L-1,甲草胺 12 8mg·L-1)处理组的最大比增长率分别为对照组的 12 38%和 31 5 8% ;骠马低浓度对普通小球藻的抑制作用不明显 ,并呈一定的生长促进效应 ,0 0 8mg·L-1浓度组普通小球藻最大比增长率为对照组的 111 4 4 % ,而高浓度则具有明显的生长抑制作用 ,并随时间推移 ,毒性逐渐减弱 .嗪草酮、骠马和甲草胺的 96hEC50 分别为 0 0 2 1、0 937和 5 5 4mg·L-1.普通小球藻对嗪草酮最敏感 ,其次为骠马和甲草胺 .3种除草剂在实验条件下对普通小球藻叶绿素a含量的影响和对普通小球藻生长的影响相似 ,表现出较好的剂量 效应关系 .
The relative sensitivities of Chlorella vulgaris to three commonly used herbicides metribuzin,puma and alachlor were determined by toxicity tests of 96-h static exposures.The results showed that the toxicities of metribuzin and alachlor to C.vulgaris increased with exposure time,and the density of C.vulgaris decreased with increasing concentrations of these two herbicides in the test media.The maximum specific growth rate of C.vulgaris exposed to metribuzin (0.24 mg·L -1 ) and alachlor (12.8 mg·L -1 ) was 12.38% and 31.58% of the control,respectively.At low concentration,puma stimulated C.vulgaris growth,but at high concentration,it inhibited the growth significantly.The maximum specific growth rate of C.vulgaris under 0.08 mg·L -1 puma was 111.44% of the control,and the toxicity of puma declined with increasing exposure duration.The EC 50 value of metribuzin,puma and alachlor was 0.021,0.937,and 5.54 mg·L -1 ,respectively.The relative sensitivities of C.vulgaris to the three herbicides decreased in the order of metribuzin,puma and alachlor.The chlorophyll a content of C.vulgaris decreased with the increasing concentrations of metribuzin,puma and alachlor.
出处
《应用生态学报》
CAS
CSCD
2004年第9期1621-1625,共5页
Chinese Journal of Applied Ecology
基金
国家自然科学基金资助项目 ( 3 0 170 162 )
关键词
嗪草酮
骠马
甲草胺
普通小球藻
毒性
叶绿素
Metribuzin, Puma, Alachlor, Chlorella vulgaris, Toxicity, Chlorophyll.