摘要
目的 研究转化生长因子 β1(TGF β1)基因在大鼠白内障发生过程中的表达。方法 给 3周龄大鼠单眼球后注射 2 0 %半乳糖生理盐水诱导白内障的发生 ,运用RT PCR方法测定在不同时相大鼠晶状体中TGF β1基因的表达。结果 半乳糖注射后晶状体上皮细胞TGF β1mRNA于 8h开始上升 ,2 4h达高峰 ,是正常对照的 4倍 ,2d后迅速下降 ,4d完全恢复到正常水平。结论 在白内障形成的早期 ,晶状体细胞中TGF β1呈高表达 ,提示TGF
Objective To investigate the expression of transforming growth factor-β_(1) (TGF-β_(1)) gene in galactose-induced cataract in rats. Methods Cataract was induced by single-retrobulbar-injection of galactose in three-week-old rats.Expression of TGF-β_(1) at different cataract phases was determined by RT-PCR. Results TGF-β_(1)mRNA began to increase at 8 hours after injection of galactose and reached fourfold that of normal levels at 24 hours.TGF-β_(1)mRNA level decreased rapidly 2 days later and returned to the normal level after 4 days. Conclusion There is an overexpression of TGF-β_(1)mRNA in early stage of galactose-induced cataract,which may play an important role in the galactose-cataractgenesis.
出处
《眼科研究》
CAS
CSCD
北大核心
2004年第5期453-455,共3页
Chinese Ophthalmic Research
基金
国家自然科学基金(39460 0 77)
贵州省科委基金 [(93)30 76]资助
