小柴胡汤及其药群配伍对角叉菜胶诱导大鼠胸膜炎模型细胞因子的影响

Effect of Xiaochaihu Decoction and Different Herbal Formulations of Its Components on Cytokines of Carrageenan Induced Pleuritis in Rats

目的探讨小柴胡汤 (柴胡、黄芩、半夏、生姜、人参、甘草和大枣 )及其药群配伍的抗炎作用 ,并探讨其可能作用机制。方法在大鼠胸腔内注射 1%角叉菜胶 0 2ml诱发胸膜炎模型。中药各组分别给予小柴胡汤不同药群配方的中药灌胃 (分别按如下组成和剂量 :柴芩 3 5 g/kg ,姜夏 3g/kg ,参草枣 4 5 g/kg ,柴芩 +姜夏 6 5 g/kg ,柴芩 +参草枣 8g/kg ,姜夏 +参草枣 7 5g/kg ,全方 11g/kg) ,正常组和模型组给予等体积的蒸馏水灌胃。连续给药 5天 ,在末次给药后 2h造模 ,造模 12h后检测胸腔渗出液体积、渗出液中白细胞计数、髓过氧化物酶 (MPO)、肿瘤坏死因子 α(TNF α)、白细胞介素 1β(IL 1β)及血清白细胞介素 8(IL 8)含量。结果与模型组比较 ,小柴胡汤及其药群配伍各组大鼠胸腔渗出液体积、渗出液中白细胞数量、MPO和TNF α含量以及血清IL 8含量均显著降低 (P <0 0 5 )。柴芩组、姜夏组、全方组胸膜炎胸腔渗出液中IL 1β含量显著降低 (P <0 0 1)。结论小柴胡汤及其药群配伍均有显著的抗炎作用 ,对胸膜炎有一定的防治作用 ;其中柴芩组、全方组、姜夏组和参草枣组作用较优。其作用机制可能与小柴胡汤及其药群配伍通过抑制TNF α、IL 1β和IL 8等细胞因子的升高 ,抑制白细胞活化。

ObjectiveTo explore the anti-inflammatory effect and possible mechanism of Xiaochaihu Decoction (XCHD) and the different herbal formulations of its components. MethodsRat model of pleuritis was established by thoracic injecting 0 2 ml of 1% carrageenan. Rats in the treated groups were medicated with XCHD (11g/kg) and the different herbal formulations of its components respectively as follows: thorowax root-scutellaria root (A, 3 5 g/kg), fresh ginger-pinellia tuber (B, 3 g/kg), ginseng-licorice root-jujube (C, 4 5 g/kg), A+B (6 5 g/kg), A+C (8 g/kg) and B+C (7 5 g/kg), and those in the normal group and the model group were given equal volume of instilled water, by way of gastrogavage for successive 5 days. Modeling was performed 2 hrs after the last medication. The amount of pleurorrhea, and leucocyte (WBC), marrow peroxidase (MPO), tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) in pleurorrhea, and serum level of interleukin-8 (IL-8) were measured 12 hrs after modeling. ResultsAs compared with the model group, all the parameters measured were lower in all the treated group (P<0 05) , and IL-1β content in pleurorrhea in the XCHD group and Group A, B and C were significantly lower (P<0 01). ConclusionXCHD and the different herbal formulations have obvious anti-inflammatory effect, showing certain preventive and therapeutic effect on pleuritis. Among the different formulations, the XCHD, A, B and C had better effects. The effects might be related to inhibiting the increase of cytokines as TNF-α, IL-1β, and IL-8 to suppress the activation, infiltration and wandering of leucocytes.