视网膜母细胞瘤的VEGF和NF-κB P65表达及意义

EXPRESSIONS AND SIGNIFICANCE OF VEGF AND NF-κB P65 IN RETINOBLASTOMA

①目的 探讨血管生成因子VEGF和细胞核因子NF κBP6 5蛋白在视网膜母细胞瘤中的表达及其与视网膜母细胞瘤生物学行为的关系。②方法 应用免疫组织化学SABC法检测VEGF和NF κBP6 5蛋白在 36例视网膜母细胞瘤中的表达情况。③结果 NF κBP6 5在正常视网膜中呈阴性表达 ,VEGF在正常视网膜中呈弱阳性表达 ,而在视网膜母细胞瘤中两者均呈阳性表达。两者在肿瘤组织细胞中的表达与在正常组织细胞中的表达差异有显著性 (F =1 5 2、1 2 3,q =4 0 .1 1、2 7.2 0 ,P <0 .0 5 )。且VEGF在视网膜母细胞瘤视神经浸润组与未浸润组的表达之间也有统计学差异 (q =1 0 .5 3,P <0 .0 5 )。 ④结论 VEGF和NF κBP6 5均参与视网膜母细胞瘤的形成 。

Objective To investigate the expressions of vascular endothelial growth factor(VEGF) and nuclear factor kappa B( NF-κB P65) in retinoblastoma, and the relationship between the expressions and biological behavior of the disease. Methods SABC immunohistochemical method was used to detect the expressions of VEGF and NF-κB P65 in 36 patients with retinoblastoma. Results No positive expression of NF-κB P65 was observed in normal retina, but there was weak expression of VEGF. Positive expressions of both were observed in retinoblastoma. The difference between VEGF and NF-κB P65 in their expressions in retinoblastoma and normal retina were statistically significant ( F=152,123;q=40.11,27.20;P <0.05). The expression of VEGF differed significantly between non-invasive and invasive optic nerves of retinoblastoma ( q=10.53,P <0.05). Conclusion VEGF and NF-κB P65 are closely related to formation of retinoblastoma. VEGF may be associated with the invasion of the condition.