缓释VEGF的可吸收性微管在TMR中的应用研究

Applied study of biodegradable capsules containing VEGF in TMR

目的 :观察血管内皮细胞生长因子结合缓释技术 ,促机械性TMR管道内血管生成的作用。方法 :利用机械钻在家兔左室前壁进行心肌钻孔 ,形成透室壁性心肌管道 ,实验组心肌管道内放入含VEGF及肝素的可吸收性多孔缓释微管 ;对照组放入不含VEGF及肝素的可吸收性多孔缓释微管。动物于术后8周处死 ,利用组织切片染色法 ,对照观察实验组和对照组TMR管道内血管生成状况。结果 :实验组TMR管道内血管生成的密度 /数量为 9.2 4± 0 .46条 /10× 4倍 ;对照组为 1.98± 0 .87条 /10× 4倍 ,两者具有非常显著性差异 ,P <0 .0 0 1。结论 :VEGF通过可吸收性多孔微管的缓释作用 ,对管道内血管的再生与重建具有非常明显的促进作用。

Objective: To research the effect of vascular endothelial growth factor (VEGF) with controlled drug release system on the angiogenesis and revascularization in the drilling TMR channels from the view of morphology. Methods: Transmyocardial channels were made by machine drilling in vivo on left ventricular wall of the rabbits. In the experimental group, the biodegradable capsules containing VEGF were put in the channels after TMR; while in control group, the capsules put in channels didn't contain VEGF .The animals in two groups were sacrificed at 8 weeks after TMR . The effect of angiogenesis and revascularization were observed, and the new vessels(NV) in channels of two groups were measured under microscope. Results: Statistical analysis demonstrated a high significant difference between the experimental and control groups in the density and quantity of new vessels(9.24±0.46NV per 10×4 field in experimental group versus 1.98±0.87 NV per 10×4 in control group, P <0.001).Conclusion: The results confirm that the good effect of angiogenesis and revascularization in the drilling channels can be received by the stimulation of VEGF through control drug release system.