咳喘落对哮喘发病过程中IL-4、IL-5的影响

Influence of“Cough and Asthma Remover”on IL-4 and IL-5 In Process of Asthma Seizure

为了探讨中药在抑制哮喘气道变应性炎症中的作用机理 ,采用双抗体夹心ELISA法和逆转录 多聚酶链反应 (RT PCR)检测咳喘落治疗哮喘过程中IL 4、IL 5含量和IL 4mRNA、IL 5mRNA表达水平的变化。结果显示 :哮喘时IL 4、IL 5含量及其基因表达明显增强 ,经咳喘落治疗后 ,其表达和产物显著降低 ,接近于正常水平 (P <0 .0 1)。提示IL 4与IL 5在哮喘发病中起着重要作用 ,咳喘落能够降低IL 4和IL 5基因转录 ,减少两者的生物合成 ,进而抑制嗜酸性粒细胞 (Eos)的聚集、活化 ,从而发挥其抗气道炎症的作用。

Inordertoinvestigatethefunctionalmecha nismofherbaldrugsinantagonizingvariantinflammation inairwaysinasthma ,thedoubleantibodymethodwith ELISAandRT PCRwasusedtotestIL 4andIL 5con tentsandchangesintheexpressionlevelsofIL 4mRNA andIL 5mRNAduringthetreatmentofasthmaby“Cough andAsthmaRemover” .TheresultsshowedthatIL 4and IL 5contentsandgeneexpressionincreasedobviously duringasthmaseizure ,andaftertreatmentwith“Coughand AsthmaRemover” ,theirexpressionandproductsremark ablydecreasedandwerealmostclosetonormallevel(P < 0 .0 1) ,indicatingthatIL 4andIL - 5playedanimpor tantroleinasthmaseizure ,and“CoughandAsthmaRe mover”candecreasegenetranscriptionofIL 4andIL 5 anddecreasetheirbiologicalsynthesisforhenceinhibiting aggregationandactivationofeosinophilicgranulocyts (EOs)toplayitsroleinresistinginflammationinairways .