彩绒革盖菌固体发酵生产木质素酶工艺优化研究

Study on the Optimization of Solid Fermentation for Ligninase Production from Coriolus versicolor

采用均匀设计U15( 5 8)和双温度培养法进行彩绒革盖菌固体发酵生产木质素酶 ,对漆酶、愈创木酚酶、多酚氧化酶的活力进行回归分析。结果表明 :应用双温度培养法进行彩绒革盖菌固体发酵生产木质素酶时 ,在自然补给氧气 ,培养基pH自然 (约 6 5 ) ,并保持环境湿度约 60 %的条件下 ,2 0d是适宜的发酵周期 ;玉米浆、麸皮、 (NH4 ) 2 SO4 、水分适宜作为固体培养基的成分 ;少量的Tween 80有利于木质素酶的生产。

In this experiment, solid fermentation of Coriolus versicolor with uniform design U 15 (58) had been performed at two different temperature degrees. The activities of laccase, guaiacolase,and polyphenol oxidase were regardedgarodedas dependant in Multivariate Regression Analysis by SPSS, and then the mathematical model of overall ligninase activity was constructed by adding activity equations of laccase, guaiacolase, polyphenol oxidase together with weights which were the quotients of corresponding total correlation coefficients and average enzyme activity values. Furthermore, the solutions to the maximal ligninase model were figured out by constraint mathematical programming. It is revealed that in ligninase production by solid fermentation with at temperature degrees from Coriolus versicolor, 20dis lis proper fermentation time for ligninase production under the condition of natural aeration, natural substrate pH(about 6.5) and an environmental humidity of 60%. Furthermore, corm syrup, wheat bran, (NH 4) 2SO 4 and water are proper substrate ingredients and a little Tween-80 is helpful for ligninase production.