摘要
以奥尼罗非鱼(Oreochromisniloticus×O.aureus)为实验材料,研究其淀粉酶、脂肪酶分布与特性。结果表明,Ⅰ(体重55.14g)、Ⅱ(体重122.82g)、Ⅲ(体重225.68g)组实验鱼肠道淀粉酶、脂肪酶活性均表现出相似规律:活性从大到小依次为前肠、中肠、后肠。实验鱼体重从55g增至122g,肠道淀粉酶活性急剧上升,实验鱼体重从122g增至225g,酶活性增幅减缓。3组实验鱼肠道各肠段淀粉酶活性相对比值(前肠、中肠、后肠)分别为1:0.95:0.52、1:0.44:0.21和1:0.50:0.24;脂肪酶活性相对比值(前肠、中肠、后肠)分别为1:0.17:0.12、1:0.34:0.14和1:0.98:0.64。3组实验鱼淀粉酶活性相对比值(Ⅰ、Ⅱ、Ⅲ组)在全肠、肝胰脏中分别为1:8.25:18.94和1:3.43:9.31;3组实验鱼脂肪酶活性相对比值(Ⅰ、Ⅱ、Ⅲ组)在全肠中为1:3.82:13.09。奥尼罗非鱼肠道、肝胰脏淀粉酶最适pH值分别为6.5~7 0和7.5;最适反应温度均为30℃;最适反应底物浓度均为0.8%。肠道脂肪酶最适pH值为7.0~7.5,最适反应温度为35℃。
Three groups of tilapia (Oreochromis niloticus ×O.aureus), average body weight in groups Ⅰ,Ⅱ and Ⅲ as 55.14, 122.82 and 225.68 g respectively, were employed to study the development and distribution of digestive enzyme in alimentary tract. The results showed that the activities of amylase in total intestine rose sharply with the body weight increasing from 55 g to 122 g, and that went up slowly with increase of body weight from 122 g to 225 g. The activities of amylase and lipase in all groups showed the same regularity as those in fore intestine, which were higher than those in middle intestine. The activities of amylase and lipase hind intestine were the lowest. The relative ratio of amylase activity among fore, middle and hind intestines in the in three groups (Groups Ⅰ,Ⅱ and Ⅲ) were 10.950.52, 10.440.21 and 10.500.24, respectively;and the relative ratio of lipase activities among fore, middle and hind intestines in the three group (Groups Ⅰ,Ⅱ and Ⅲ) were 10.170.12,10.340.14 and 10.980.64. The relative ratio of amylase activities in the three group (Ⅰ,Ⅱ,Ⅲ) in hepatopancreas and total intestine were 13.439.31 and 18.2518.94 respectively;and the relative ratio of lipase activities in the three groups (Ⅰ,Ⅱ,Ⅲ) in total intestine were 13.8213.09. A group of tilapia (Oreochromis niloticus ×O.aureus) (average body weight as 111.81 g) were employed to study properties of digestive enzyme in alimentary tract. The results showed that the optimum pH for amylase in intestine and hepatopancreas were 6.5~7 and 7.5, respectively, and the optimum temperature for amylase in intestine and hepatopancreas was 30℃, and the optimum substrate concentration for amylase in intestine and hepatopancreas was 0.8%. The optimum pH and temperature for lipase were 7.0~7.5 and 35 ℃, respectively.
出处
《中国水产科学》
CAS
CSCD
北大核心
2004年第5期473-477,共5页
Journal of Fishery Sciences of China
基金
中国水产科学研究院重点科研计划项目(2001-53)
南京农业大学青年创新基金(Y200206)