黄芪和多巴胺对实验性急性肾衰大鼠的NO和ET影响

Effect of Astragalus and Dopamine on Changes of Blood and Renal Tissue Contents of NO, ET in Experimental Rat Models of Acute Renal Failure

目的 :探讨静脉或肾脂肪囊内注射黄芪和多巴胺对实验性急性肾衰大鼠的血清NO及肾组织NO和ET的影响。方法 :SD大鼠腹腔注射LPS建立大鼠内毒素休克模型后 ,分别在静脉或肾脂肪囊应用黄芪和多巴胺 ,在 4、8、12、16h测定血清内NO及肾组织内NO和ET的浓度。结果 :肾脂肪囊内联合应用黄芪与多巴胺组血清内NO及肾脏内NO和ET浓度增加较内毒素组明显为少 (P <0 0 5 ) ,在 12h至实验结束 ,肾脂肪囊组肾脏内NO和ET浓度较静脉组明显降低 (P均 <0 0 5 )。结论 :肾脂肪囊内联合应用黄芪与多巴胺 ,可以降低急性肾衰大鼠肾脏内NO与ET的浓度 ,且较静脉给药组明显。

Objective To explore the effect of intraventous or intra-renal-capsular administration of astragalus and dopamine on the serum NO and renal tissue NO、 ET contents in rat models of acute renal failure. Methods Experimental rat models of acute renal failure induced by intraperitoneal injection of E.Coli endotoxin (lipo-polysacchride) were prepared (n=60). Treatment with astragalus and dopamine was administered via either intravenous on intra-renal-capsular route (n=20 in each group). Serum NO and renal tissue NO(with nitric acid reductase method) , ET (with RIA) contents were determined at 4, 8, 12, 16h after injection of endotoxin. Twenty shock models were left untreated and addifional twenty rats receiving saline injection only served as controls. Results In the intravenously treated group, the increase of serum NO and renal tissue NO、ET contents were significantly less than those in the untreated group (P<0.05). In the group treated via the intracapsular route, the increase of renal tissue NO and ET contents were much less than those in the intravenous group at 12 and 16h (P<0.05). Conclusion Combined treatment with astragalus and dopamine could abate the abnormally high renal tissue contents of NO and ET after endotoxin shock in experimental rats and treatment with intra capsular administration seems to be more effetive.