摘要
经多年试验研究.筛选出适于葡萄茎尖脱毒培养及继代繁殖的广谱性系列培养基.它与一般常用培养基相比.可缩短培养时间12～19d.该系列培养基对不同葡萄品种的培养效果有一定的差异,但脱毒效果差异不明显,对葡萄扇叶病毒和卷叶病毒的茎尖脱毒率分别达到86.6％和84. 4％.试验证明,茎尖的长短与脱毒率成反比,与再生株的存活率成正比.
A series of broad spectrum media for stem tip tissue virus - free culture and propagation of grapevine was prepared-These media could shorten the culture time by 12 -19 days as compared with the media used commonly. The series of media appeared different in culture effect on the different grapevine varieties. The elimination effect of the virus in grapevine showed little different with the varieties. The elimination rate of GFV and GLRV from the grapevine stem tip reached up to 86. 6% and 84. 4% .respectively. The result indi cated that the length of the stem tip varied directly with the rate of virus elimination and in versely with the survival rate of the plant.
出处
《华北农学报》
CSCD
北大核心
1993年第2期69-72,共4页
Acta Agriculturae Boreali-Sinica
关键词
葡萄
脱毒
培养基
组织培养
繁殖
Grapevine
Elimination of virus
Medium
Tissue culture
