运用单向灌流模型研究莪术油中三成分对大鼠在体肠的吸收

Absorption of zedoary oil in rat intestine using in situ single pass perfusion model

目的 研究莪术油中三成分对大鼠在体肠的吸收情况。方法 运用单向灌流模型并采用HPLC法(配备二极管阵列检测器)同时测定莪术油中三成分在灌流液中的浓度。结果 灌流液中莪术油浓度为0.4,0.8和1.2mg·mL-1时,莪术醇、莪术二酮和吉玛酮表观吸收系数均较低,且均无显著性差异(P>0.05),各肠段的表观吸收系数也无显著性差异(P>0.05);吉玛酮和莪术二酮二者在体肠吸收情况无显著性差异(P>0.05),但均高于莪术醇,且存在显著性差异(P<0.01)。结论 灌流液中莪术油在0.4—1.2 mg·mL-1时,三成分的吸收无自身浓度抑制作用,在肠黏膜的转运均为被动扩散过程,且三成分无特殊的吸收窗。

Aim To study the absorption of zedoary oil in intestine of rat. Methods In situ single pass perfusion model was used and the concentrations of three components in perfusate were determined by HPLC in combination with diode array detection. Results The Papp s of curcumol, curdione and germacrone were all low and had no significant difference ( P > 0. 05 ) at zedoary oil concentration of 0. 4, 0. 8 and 1. 2 mg · mL-1 in transmucosal fluid or in four different regions of intestine of rat [ duodenum, jejunum, ileum, colon ]. The absorption rates of germacrone and curdione were faster than curcumol's in this study. Conclusion The zedoary oil concentration in transmucosal fluid had no significant effect on the Papps within the scope of 0. 4 - 1. 2 mg · mL-1 . The absorption of curcumol, curdione and germacrone showed the passive diffusion process, and didn't contain a special absorption window.