莪术多糖的分离提取及其生物学活性研究

FRACTIONATION OF POLYSACCHARIDBS FROM CURCUMA ZEDOARIA ROSC AND ITS BIOACTIVITIES

目的:从莪术饮片中分离多糖,并研究其生物学活性。方法:莪术饮片的热水浸提液经浓缩、醇析、活性碳吸附除色素、Savage 法除蛋白得其粗多糖,经DEAE-52柱层析,分离各组分。苯酚-硫酸法测多糖含量。采取腹腔注射与口服两种途径在小鼠测其生物学活性。结果:莪术饮片中总糖的含量为33.12%,多糖提取率为1.938%。莪术多糖可以提高小鼠的脾指数、淋巴细胞转化率;提高血液中超氧化物歧化酶的活力;具有抑瘤作用;体外具有抗羟自由基的能力。腹腔注射时抑瘤率明显高于口服途径,并呈现剂量依赖性。结论:莪术多糖可以提高小鼠的免疫调节能力,表现出抗氧化活性;并具有较高的抗H22肝癌腹水瘤的能力。

Objective: To extract the polysaccharides of Curcuma zedoaria Rosc (PCZ) and study its bioactivities. Methods: The crude polysaccharides were extracted from herbal slice of Curcuma zedoaria Rosc with hot water ,and precipitated by ethanol and savage deproteinization and depigmentation with activated charcoal. The constituents were isolated by column chromatography and the total polysaccharides content was determined. Experiments of immune function,anti-oxidation and anti-tumor activity of KM mice and in viro were studied. Results: The content of total polysaccharides of Curcuma zedoaria Rosc was 33.12%. and the extraction rate was 1.938% . It could raise spleen cell proliferation and transformation rate of lymphocyte and SOD activity in blood of mice. A higher antitumor effect of the polysaccharides by injection was observed dose-dependently. Conclusion: PCZ could raise the immune and anti-oxidative activities and then had a higher antitumor effect.