犬全氟异丁烯吸入性急性肺损伤模型的建立

The establishment of acute lung injury model in dogs induced by perfluoroisobutylene inhalation

目的 建立全氟异丁烯 (perfluoroisobutylene ,PFIB)致犬吸入性急性肺损伤 (acutelunginjury ,ALI)模型。方法 对麻醉犬进行气管插管、颈动脉插管 ,采用气管联接方式吸入中毒 ,毒气室PFIB浓度 0 3mg/L ,吸入 3 0min。检测中毒犬动脉血气、肺泡灌洗液中总蛋白含量和肺湿 /干比的变化。结果 中毒犬染毒后 12h动脉血氧分压 (PaO2 )明显降低 ;2 4h降至 5 2mmHg ;尸检肺部大量渗出、充血、水肿 ,支气管肺泡灌洗液中总蛋白含量高达 162 0mg/L ,湿肺体比、肺湿干比和肺含水量明显升高。结论 本模型符合ALI诊断标准 。

Objective To establish the dog model of acute lung injury(ALI) induced by perfluoroisobutylene(PFIB) inhalation. Methods The anesthetized dogs inhaled PFIB through trachea connection at the concentration of 0 3 mg/L for 30 min. Blood samples were obtained by carotid artery cannula. Artery blood gas, total proteins concentration of bronchoalveolar lavage fluid(BALF), and lung wet/dry weight ratio(Lw/Ld) were measured. Results The pressure of artery oxygen (PaO 2) decreased significantly 12 hours after PFIB exposure, and further lowered to 52 mmHg 24 hours after exposure. Total proteins concentration in BALF increased to 1 620 mg/L, and lung Lw/Ld significantly increased as well. Systematical autopsy showed obvious bleeding, hyperemia and edema in the lungs. Conclusion The dog model of ALI induced by PFIB inhalation was coincided with the diagnosis standard of ALI.