黄连煎剂对家兔离体肠段运动性能的影响

Effects of Coptis Decoction on Activity of In Vitro Intestine of Rabbit

采用离体器官试验法,将同一家兔离体十二指肠和空肠置于恒温通气麦氏浴皿中,向盛有台氏液的麦氏浴皿中逐次加入1mL不同浓度(0.125g／mL,0.25g／mL,0.5g／mL,1.0g／mL)的黄连煎剂,通过BL-New Century生物信号采集处理系统,记录用药前后肠段运动幅度变化、张力变化及运动频率变化情况。观察不同浓度黄连煎剂对家兔离体十二指肠和空肠运动性能的影响,并探讨其药理作用的机理。结果表明十二指肠和空肠用浓度为0.125g／mL处理后,运动幅度显著增大(0.01<p<0.05);十二指肠张力显著增大(p<0.01),空肠张力增加不显著(p>0.05);运动频率都加快。其它三种浓度处理后,十二指肠,空肠运动幅度都显著减小(p<0.01);张力显著减小(p<0.01);频率也都减慢。结论:低浓度黄连煎剂(c<0.125g／mL)对家兔离体小肠起兴奋作用,高浓度(c>0.25g／mL)起抑制作用。

Adopt experiment of in vitro organ; add 1mL but different concentration (0. 125g/mL. 0. 25g/mL. 0. 5g/mL. 1. 0g/mL) Coptis Decoction in constant temperature Maishi container. Through BL - New century creature signal collection and management system, scan and write down the activity extent, tension and frequency to observe the effects of different concentrations on the activity of in vitro Duodenum and Jejunum of rabbit and study the causes of medicine. It shows the result; When using the concentration at 0. 125g/mL, both Duodenum and Jejunum's activity extents, tension and frequency enlarge, The test of Duodenum is significant (0.01 <p < 0.05), but Jejunum is not significant (p >0.05). At 0. 25g/mL, 0.5g/mL, 1.0g/mL, both duodenum and jejunum's activity extents decrease, and the test is more significant (p <0. 01) , The tension and frequency also decrease, and the test is more significant ( p <0.01). Conclusion; ard the lower concentrations of Coptis decoc-tion(c<0.125g/mL) excite the in vitro small Intestine of rabbit while the higher ones(c >0.25g/mL) inhibit it.