三袖套血管吻合法行大鼠异位小肠移植

Tri-cuff vascular anastomosis for the heterotopic small bowel transplantation in rat

目的改进大鼠小肠移植血管吻合术式,以缩短移植肠缺血时间,简化手术操作,提高手术成功率。方法动脉吻合采用供者带肠系膜上动脉的腹主动脉段两端与受者的腹主动脉袖套吻合,静脉吻合采用供者的门静脉与受者的左肾静脉袖套吻合,即三袖套吻合法。结果共进行70次手术,成功65次,手术成功率92.9%;整个手术用时2～3h,其中动脉吻合用时(5±2)min,静脉吻合用时(2±1)min,动静脉吻合用时(8±2)min。结论三袖套血管吻合法可简化大鼠小肠移植手术,缩短手术时间。

Objective To improve the vascular anastomosis of the rat small intestinal transplantation, reduce the ischemia of transplanted intestine and make it apt to survive, simplify the operation procedure, elevate the operation success rate. Methods Artery anastomosis was done by employing the cuff anastomosis between a segment of donor abdominal aorta with superior mesenteric artery and recipient abdominal aorta, and venous anastomosis by employing the cuff anastomosis between donor's portal vein and recipient's left renal vein, namely Tri cuff anastomosis. Results Sixty six out of 70 transplant operation cases survived with the operation success rate being 92.9 % . Total operation time was about 2 3 h . The average time for the arterial anatomosis and venous anastomosis was (5±2) min and (2±1) min, respectively. Conclusion The method simplifies the rat small intestine transplantation, shortens the operation time and improves the operation survival rate.