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两种生长因子对牙周膜细胞表达骨保护因子的影响 被引量:2

Insulin-like Growth Factor-Ⅱ and Basic Fibroblast Growth Factor Affect Periodontal Ligament Cells Expressing Osteoprotegerin in vitro
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摘要 目的 观察胰岛素样生长因子-Ⅱ(IGF-Ⅱ)和碱性成纤维细胞生长因子(bFGF)对牙周膜细胞增殖及分泌骨保护因子(OPG)的影响。方法 培养牙周膜细胞并形成单细胞克隆,培养基中加入bFGF和IGF-Ⅱ,MTT法检测牙周膜细胞增殖水平,Sandwich ELISA测定培养上清液中OPG含量。结果IGF-Ⅱ促进牙周膜细胞增殖但降低细胞OPG的表达量(P<0.05)。bFGF也促进牙周膜细胞增殖(P<0.05),但对细胞表达OPG的影响作用不明显(P>0.05)。结论 应用细胞因子促进牙周再生时应考虑其对牙周骨组织平衡的影响。 Objective This study was carried out to investigate the effects of insulin-like growth factor-Ⅱ(IGF- Ⅱ ) and basic fibroblast growth factor (bFGF) on osteoprotegerin(OPG) secretion of periodontal ligament cells (PDLCs) .Methods Healthy human premolars extracted for orthodontic reasons from 12-14 years old donators were obtained, and periodontal tissues were collected and cultured to obtain PDL cells. Primary or first passage PDLCs were cloned by means of limited dilutions. PDLCs with os-teoblastic phenotypes were characterized as follows: Alkaline phosphatase activity, collagen Ⅲ production and bone-like nodules formation. IGF- Ⅱ and bFGF were added into culture media and their effects on PDLCs proliferation and OPG secretion were observed. The OPG concentrations in cell culture supernatants were detected by sandwich ELJSA. Living cell numbers were demonstrated by MTT test. The average levels of OPG secretion by a single cell were calculated by dividing OPG concentration with MTT-test result. Results Both IGF- Ⅱ and bFGF upregulated the mtt values ( P < 0.05 ), but IGF- II downregulated the opg/mtt values ( P < 0.05), whereas bFGF had no significant effect on opg/mtt values ( P > 0.05). Conclusion IGF- II enhances the proliferation of PDL cells but prohibits OPG secretion. Although bFGF has the same effect on the proliferation of PDL cells, it has no effect on OPG secretion. Before cytokines were used to enhance periodontal regeneration, their effects on local bone balance should also be studied.
出处 《华西口腔医学杂志》 CAS CSCD 北大核心 2004年第5期366-369,共4页 West China Journal of Stomatology
关键词 牙周膜 细胞培养 碱性成纤维细胞生长因子 胰岛素样生长因子 periodontium cell-cultured basic fibroblast growth factor insulin-like growth factor
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  • 1Kong YY, Yoshida H, Sarosi I, et al. OPGL is a key regulator of osteoclastogenesis, lymphoctye development and lymph-node organogenesis[J]. Nature, 1999, 397(6717): 315-323.
  • 2Lacey DL, Timms E, Tan HL, et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation[J]. Cell, 1998, 93(2): 165-176
  • 3Yasuda H, Shima N, Nakagawa N, et al. A novel molecular machanism modulating osteoclast differentiation and function[J]. Bone,1999, 25(1): 109-113.
  • 4Horwood NJ, Kartsogiannis V, Quinn JM, et al. Activated T lymphocytes support osteoclast formation in vitro[J]. Biochem Biophys Res Commun, 1999, 265(1): 144-150.
  • 5Sakata M, Shiba H, Komatsuzawa H, et al. Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells[J]. J Bone Miner Res, 1999, 14(9): 1486-1492.
  • 6Somerman MJ, Archer SY, Imm GR, et al. A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro[J]. J Dent Res, 1988, 67(1): 66-70.
  • 7Hou LT ,Yaeger JA. Cloning and characterization of human gingival and periodontal ligament fibroblasts[J]. J Periodontol, 1993, 64(12): 1209-1218.
  • 8Teng YT, Nguyen H, Gao X, et al. Functional human T-cell immunity and osteoprotegerin ligand control alveolar bone destruction in periodontal infection[J]. J Clin Invest, 2000, 106(6): 749-752.
  • 9Crotti T, Smith MD, Hirsch R, et al. Receptor activator NF kappaB ligand (RANKL) and osteoprotegerin (OPG) protein expression in periodontitis[J]. J Periodontal Res, 2003, 38(4): 380-387.
  • 10Liu D, Xu JK, Figliomeni L, et al. Expression of RANKL and OPG mRNA in periodontal disease: possible involvement in bone destruction[J]. Int J Mol Med, 2003, 11(1): 17-21.

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  • 1柴本甫,中华实验外科杂志,1985年,4卷,157页

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