急性脑梗死不同时期红细胞内GSH、MDA含量变化及其临床意义

Determination of erythrocyte glutathione level in patients with acute cerebral infarction during various periods

目的:探讨还原型谷胱甘肽(GSH)在缺血性脑卒中的抗氧化作用。方法:应用紫外分光光度法对98例急性脑梗死患者发病后不同时期的红细胞内谷胱甘肽(RBC鄄GSH)、丙二醛(RBC鄄MDA)含量以及红细胞变形指数(RBC鄄DI)进行观测。结果:急性期RBC鄄GSH含量明显降低(P<0.001)。RBC鄄MDA含量明显增高穴P<0.01雪。其中发病后3天最为显著,第7天开始恢复,至第14天RBC鄄GSH含量恢复正常。经直线相关分析,急性期RBC鄄GSH含量与RBC鄄MDA含量、患者的病情严重程度呈负相关,与RBC鄄DI呈正相关。结论:RBC鄄GSH含量变化能够反映急性脑梗死继发的过氧化—抗氧化功能失调的程度,可为诊断脑梗死病情轻重的临床生化指标之一。

Objective: To study the effects of reduced glutathoine (GSH) on the antioxidation following acute cerebral infarction. Methods: The levels of erythrocyte glutathione (GSH) and malonic dialdehyde (MDA) were determined in 30 healthy subjects and 98 patients with acute cerebral infarction during various periods using ultraviolet spectrophotometry. The correlations between GSH and other parameters were also analyzed. Results: Compared with the controls, the patients with cerebral infarction showed a lower GSH level and a higher MDA level at the acute stage, particularly on the third day after onset (P<0.001). GSH level negatively correlated with MDA level (P<0.05) but positively correlated with erythrocyt deformability indexs. Conclusion: We conclude that the level of GSH in the erythrocyte could reflect the dyfunctional degree of peroxidation-antioxidation following acute cerebral infarction.